qPCR Probe 2x LyoCake Master Mix simplifies your PCR setup and can be stored at room temperature. A cool chain is not needed anymore. The Master Mix is freeze-dried and dissolves within a few seconds after addition of the included rehydration buffer.
Detail
qPCR Probe 2x LyoCake Master Mix simplifies your PCR setup and can be stored at room temperature. A cool chain is not needed anymore. The Master Mix is freeze-dried and dissolves within a few seconds after addition of the included rehydration buffer.
After rehydration of the LyoCake, only primers, probes and template need to be added as the 2x Master Mix contains all components for a successful and reliable qPCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors. The robustness of qPCR performance allows the application of this mix in a wide range of PCR applications.
For research use and further manufacturing.
In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
Other Products
Plasmid Purification Magnetic Beads (RNA Depletion)
Product Info
Document
Product Info
Plasmid Purification Magnetic Beads (RNA Depletion)
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
We have developed a simple reagent to completely remove RNA contamination in the isolated plasmid samples using Solid Phase Reversible Immobilization (SPRI) beads. SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. Our Plasmid Purification Magnetic Beads (RNA Depletion) combines BioDynami’s proprietary chemistries with the reversible DNA-binding properties of SPRI magnetic beads. The reagent removes RNA and recovers the plasmid in the same step. Moreover, unwanted components such as salts, dNTPs, proteins, enzymes, and other impurities can also be removed simultaneously.
Plasmid can be used for downstream applications such as enzymatic digestion, transformation, transfection and molecular cloning etc. The beads can be an effective and inexpensive reagent for bacterial RNA depletion for routine plasmid purification.
Features
Effective depletion of bacterial RNA by RNase
High recovery rate of plasmid DNA by magnetic beads
Removal of unwanted components and impurities
Simple and fast beads-based protocol
Document
Plasmid isolation from bacterial cultures is one of the most popular techniques in biomedical research and pharmaceutical industries. However, it is common that the isolated plasmid DNA is usually contaminated with varied degrees of host RNA. Plasmid purification is necessary to reduce the impact on downstream applications by removing RNA contamination.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications
RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Cell, Animal tissue, Plant tissue
Sample amount
Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
Fast – several samples can be extracted in 40 minutes
High applicability – samples including animals, plants, bacteria, cells, etc.
High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
Safe – no phenol chloroform extraction and no use of Trizol reagent
Kit Contents
Contents
R431102
R431103
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
100
2 x 250
2ml Collection Tubes
100
2 x 250
Proteinase K
48 mg
240 mg
Protease Dissolve Buffer
5 ml
15 ml
Buffer RLC
40 ml
200 ml
Buffer RWC
20 ml
80 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
60 ml
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Permagen’s 15 mL Centrifuge Tube Magnetic rack is designed for magnetic bead separations from up to six, 15 mL Centrifuge tubes
Accommodates any common 15 mL Centrifuge Tubes
Beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
