GL21M max. capacity can hold 800ml bottles 4pcs, max speed over 20000rpm Model: GL21M
Brand: Yingtai
Code: 8421199090
Detail
GL21MC high speed refrigerated centrifuge
GL21MC high speed refrigerated centrifuge Application:
Widely used in the area of animal and plant molecular biology, cell biology, clinical applications, bio-engineering, mass preparation and blood, protein precipitation, Separating the biology, viruses, sub-cell, cell and the chemicals in the cell.
Features:
1.Brushless frequency motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration. 3.Import compressors fluorine free, double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control.. 4. Microprocessor control system, the 10 inches LCD display indicates all the parameters in running, 5. There are more than 20 kinds of rotors for your choice, especial the continuous rotors which help you purify a large number of test solution continuously. 6. There are 30 kinds of program and 10 kinds of accelerating and decelerating speed for your choice. 7.ω2t centrifugation is allowed, which make you have the ideal centrifugation result. 8. 20 kinds of rotors for your choice, especial the continuous rotor help you purify a large amount of test solution continuously. 9. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. 10. The centrifuge body is made of high quality steel, safe and reliable. 11. 3 tiers protection steel cover.
Technical parameters:
Max speed
21000r/min
Max RCF
46140*g
Time range
1~99H59min
Speed accuracy
±50r/min
capacity
6*500ml
Noise
≦65dBA
Weight
235KG
Dimension
865*728*1240mm
Packaged weight
285KG
Packaged dimension
1000*850*1510mm
Temperature range
-20~40℃
Temperature accuracy
±1℃
Power
AC110/220V, 50-60HZ,35A
Rotor identification
Automatic identification
Package
wooden box
Matched Rotors for GL21MC:
Order No.
Rotor
Max speed (r/min)
Max capacity (ml)
Max RCF (*g)
30108
Fixed rotor
21000
12*10ml
44440
30117
Fixed rotor
20000
16*10ml
46140
30111
Fixed rotor
20000
6*50ml
44700
30118
Fixed rotor
20000
8*30/20ml
42040
30119
Fixed rotor
15000
8*50ml
27540
30112
Fixed rotor
15000
6*70ml
25480
30120
Fixed rotor
12000
8*100ml
19610
30121
Fixed rotor
11000
4*300ml
18120
30122
Fixed rotor
10000
6*300ml
15730
30123
Fixed rotor
10000
6*500ml
18340
30128
Continuous centrifugal rotor
17000
1000ml
31390
30129
Continuous centrifugal rotor
14000
1800ml
21710
30130
Continuous centrifugal rotor
8000
3000ml
9450
30131
Continuous centrifugal rotor
8000
1800ml
8600
30132
Interval rotor
17000
1000ml
31390
30133
Interval rotor
12000
1200ml
19350
30134
Interval rotor
10000
3000ml
14890
30135
Vertical rotor
20000
16*5ml
38340
Vertical rotor
18000
8*30ml
32650
30180
Swing rotor
15000
4*5ml
21670
30181
Swing rotor
13000
4*30ml
23580
30182
Swing rotor
10000
4*50ml
16240
30694
Bucket rotor
4000
4*4*96holes
2840
30696
Swing rotor
4000
4*500ml
3380
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Product Info
Document
Product Info
Overview
Isolate high quality, high yield plasmid DNA
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. The RNase vial should be stored at -20°C upon arrival. Once RNase has been added to the Resuspension Solution AZ the solution should be stored at 4°C. This kit is stable for 1 year after its date of shipment.
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Resuspension Solution AZ
12 mL
60 mL
20 mL
100 mL
12 mL
2 x 20 mL
Lysis Buffer N
40 mL
80 mL
40 mL
2 x 80 mL
40 mL
2 x 40 mL
Buffer TN
20 mL
130 mL
55 mL
2 x 130 mL
20 mL
1 x 55 mL 1 x 20 mL
Wash Solution E
12 mL
2 x 18 mL
–
–
–
–
Elution Buffer K
8 mL
30 mL
–
–
8 mL
2 x 8 mL
Wash Solution J
–
–
25 mL
3 x 25 mL
–
–
Elution Buffer J
–
–
24 mL
120 mL
–
–
RNase A
1 vial
1 vial
1 vial
1 vial
1 vial
1 vial
Magnetic Bead Suspension
–
–
–
–
1 x 1.1 mL
4 x 1.1 mL
Spin Columns
50
250
–
–
–
–
Collection Tubes
50
250
–
–
–
–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)
–
–
4
20
–
–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Details
Specifications
Features
Specifications
Concentration
50 mg/ml
Appearance
Suspension of dark brown particles
Surface functional group
Si-OH, Silanol
Dispersibility
Monodisperse,spherical
Particle size
0.2-1.5 μm
Preservation conditions
Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth.
Magnetic response speed
20 seconds
Settling velocity
>3 minutes
High salt mediated binding
>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding
2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding
The recovery of DNA/RNA was up to 85%
DNase/RNase
Not detected
DNA residue
Not detected
Recommended application
Plasmid extraction,gel DNA recovery, genomic DNA extraction, RNA extraction, viral nucleic acidextraction, circulating DNA isolation
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
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The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
Document
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Human Papillomavirus (HPV) 6/16 TaqMan PCR Detection Kits
Product Info
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Product Info
Overview
Detection kits for the HPV 6/16
Available in TaqMan format for analysis
More than 70 types of human papillomavirus (HPV) have been identified, and are generally classified as high-risk or low-risk depending on their relationship or lack of relationship with cancer and high-grade cervical intraepithelial neoplasia (CIN 2-3). HPV viruses are predominantly sexually transmitted and high-risk HPV types are a major risk factor for development of cervical cancer. HPV 16 has been considered as a high-risk cancer associated HPV type. The low-risk HPV type 6 has been associated with the presence of genital warts. There are many other low-risk HPV types that are not associated with genital warts or cervical cancer. Until now, HPV cannot be cultured in vitro, and immunological tests are inadequate to determine the presence of HPV cervical infection. On the other hand, biopsies can be analyzed by nucleic acid hybridization to directly detect the presence of HPV DNA.
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Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
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Primer and Probe mix
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Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
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For research use only and NOT intended for in vitro diagnostics.
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