1000ml Tube (High Speed) 

Description

Specifications

Mucin 1 (MUC1) is a membrane-bound glycoprotein involved in a number of protective and cell-signaling functions, including cell-cell adhesion, proliferation, motility, invasion, and survival. Overexpression of MUC1 is clinically indicated in breast carcinomas, papillary thyroid carcinomas, and thymic carcinomas, and reports have named MUC1 as a useful marker for differentiating thymic carcinoma from type B3 thymoma. The expression of MUC1 is correlated with the grade of malignancy in thymic epithelial tumors, and loss of MUC1 expression has been associated with reactive gastropathy. MUC1 is not expressed in normal human epidermis, but has been detected in the epidermis of psoriatic plaques of biopsies from patients diagnosed with psoriasis vulgaris.

DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-NHS Ester is a very popular amine-reactive click chemistry reagent. It can be used to modify an amine-containing molecule in organic solvents (limited solubility in aqueous media). It reacts with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic pH to form covalent bonds. The low mass weight will add minimal spacer to modified molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Description 

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences. 

Features

• 5’→3’ DNA polymerase activity 
• 3’→5’ exonuclease (proofreading) activity
• Suitable for GC-rich templates 
• High reaction rate: 7 seconds/kb 
• High fidelity: 70 times higher than Taq polymerase 
• Generates blunt end amplicons  
• Vast elongation capability (up to 40 kb) 
• Thermo-stable for more than 10 hrs at 95°C 

Storage

[TF3000] G-HiFi™ DNA Polymerase

-20°C for 24 months

The G-HiFi™ DNA Polymerase is a new genetically modified, recombinant DNA polymerase suitable for GC-rich templates that are difficult to amplify. The fidelity of G-HiFi™ DNA Polymerase is 70 times higher than that of Taq DNA polymerase. The high extension rate of G-HiFi™ DNA Polymerase is achieved by blending the DNA polymerase with an elongation enhancer. The optimized 5X G-HiFi™ Buffer includes special ingredients that suppress non-specific amplification as well as plateau effect produced by conventional PCR. With the optimized 5X G-HiFi™ Buffer, G-HiFi™ DNA Polymerase is capable to amplify most templates, such as longer targets (up to 40 kb from lambda DNA) and that contain GC-rich sequences.