Description

Attogene’s Human IgG/IgM universal fluorescent lateral flow assay kit is a ready-to-use, universal test strip, which is based on the lateral flow technology that uses 655nm Emission Quantum Dot particles containing streptavidin to conveniently capture biotinylated antigens. The device is designed to easily develop qualitative or quantitative rapid test systems for detection of anti-human IgG and IgM antibody that react to the any antigen that can be biotinylated (i.e. viral antigen, autoimmune antigen) and is easily customizable providing every laboratory with the possibility to perform assay feasibility.

Formats (fluorescent broad range UV light excitation range of 300nm to 400nm, 610nm emission) Streptavidin conjugate pad):

Antibody tests are a method of choice to determine if a person has been exposed to a pathogen or not. They are also incredibly valuable in the detection of autoantibodies that can be found in human autoimmune disorders. In this test, a biotinylated antigen (User supplied) is mixed with a biotinylated rabbit IgG (bind to goat anti rabbit control line) and sample (human sera or plasma) is simply mixed into with the specially designed assay running buffer in a well of the supplied 96-well plate, mixed and is then added to the sample port of the cassette. Generally, the reaction is complete in 10-15 minutes. It is very important to note that the relative stoichiometry between the biotinylated antigen, biotinylated rabbit IgG added, and the streptavidin gold is critical for assay optimization. The appropriate concentration of biotinylated antigen to use with strips is dependent upon the purity and sequence and a standard curve can be used to determine the relative ratio (generally between 1ng-100ng per test). A positive control line (biotin-rabbit IgG) antibody will bind to the goat anti rabbit (GAR) line on the test to ensure the assay is running appropriately.

Our most popular ring magnet plate. All of the features of our T480 magnet above with the added security of a cushion base  For faster separations you can upgrade to the MSP500R which comes with all of the same features, just stronger magnets Like our ring magnet plates above, this plate comes in two magnet strengths. Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSP480R / MSP500R

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• 4 mm bead free ring at bottom of each well for easy removal
• Integrated cushion for precise pipetting and maximum recovery
• Made in USA 
• For Research use only

Compatibility

• Round bottom plates 
• Pyramid bottom plates 
• PCR Plates
• MSP480R – Up to 1000 µL volumes
• MSP500R – Up to 2000 µL volumes
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

MSP480R Maximum – 1 mL 

Minimum – 30 µL

MSP500R Maximum – 2 mL 

Minimum – 30 µL

Our most popular ring magnet plate. All of the features of our T480 magnet above with the added security of a cushion base For faster separations you can upgrade to the MSP500R which comes with all of the same features, just stronger magnets Like our ring magnet plates above, this plate comes in two magnet strengths. Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

Introduction

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.

Details

Specifications

Features	Specifications
Main Functions	Isolation gDNA from biological sample and remove host DNA
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture medium, swab, parasitic blood, tissue, sputum, etc.
Sample amount	Blood sample, homogenate, plasma, brain effusion, swab immersion solution, centrifuged concentrated liquid, etc.:0.5-1ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica Column purification. This product efficiently depletes human and animal host DNA and yields enriched bacterial DNA. An optimized combination of mechanical and chemical lysis allows efficient disruption of bacterial cells. Target DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High concentration – the host DNA is digested by dnase, without contamination of host DNA
• High recovery – DNA can be recovered at the level of PG
• Good repeatability – silica technology can obtain ideal results every time
• Wide applicability – it can be used in blood, tissue, intestinal contents and other samples

Kit Contents

Contents	D314802	D314803
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	50	2 x 125
2ml beads Tubes	50	250
Buffer DRB	15 ml	60 ml
Buffer ES	6 ml	30 ml
Reagent DX	0.5 ml	1 ml
Buffer DL	30 ml	120 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
DNase I (Powder)	6 mg	30 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability

DNase I and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

This product provides an easy-to-use workflow for selective isolation of bacterial DNA from samples that are intrinsically rich in host DNA, such as body fluids or swabs. The method is specific for the identification of intact bacteria so it prevents false results due to nucleic acids from dead bacteria. The Kit allows isolation of enriched bacterial DNA suitable for a variety of applications, including qPCR and whole metagenome or 16S rRNA gene sequencing.