Introduction

MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 50-100 mg plant, fungal tissue
Applications	PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional economic plant samples
Sample amount	Fresh / frozen plant samples:≤ 100mgDried plant / seed samples:≤ 20mg
Elution volume	≥50μl
Time per run	≤60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA was eluted by Elution Buffer.

Advantages

• Safe – require no phenol or chloroform extraction
• Fast – several samples can be extracted in 60 minutes
• High purity – high quality DNA, completely remove inhibitors

Kit Contents

Contents	D635101	D635102	D635103
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure Particles	1.7 ml	4 ml	18 ml
RNase Solution	0.6 ml	1.2 ml	6 ml
Buffer SPL	30 ml	60 ml	300 ml
Buffer PS	10 ml	20 ml	100 ml
Buffer GW1*	26 ml	53 ml	220 ml
Elution Buffer	10 ml	30 ml	120 ml

Storage and Stability

RNase Solution and MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

MagPure Plant DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and tissues.The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo.Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridization, and transgenosis detection.

Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG4-alcohol is a Click Chemistry reagent that can react with azide-bearing compounds or biomolecules; copper is needed as a catalyst. The PEG units help increase the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for high throughput PCR 

Magnetic beads are pulled high up onto well wall to help eliminate accidental bead pellet disruption 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

P384

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• High throughput applications 
• Made in USA 
• For Research use only

Compatibility

• Full- skirt 384 well PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 39 µL 

Minimum  – 10 µL 

The Permagen 384-well post magnet plate was designed for use in manual or automation applications for high throughput PCR