The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons Flat Bottom Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block can hold flat bottom plates and other flat labware at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
| Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
|---|---|---|---|---|---|
| 55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
| 35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
| 17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Maximum Amount of Starting Material | |
| Animal Cells | 1 x 106 cells |
| Animal Tissues | 10 mg |
| Blood | 100 μL |
| Plasma/Serum | 150 μL |
| Bacteria | 1 x 109 cells |
| Yeast | 1 x 108 cells |
| Fungi | 40 mg |
| Plant Tissues | 40 mg |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yields* | |
| HeLa Cells (1 x 106 cells) | 15μg |
| E. coli Cells (1 x 109 cells) | 50 μg |
* average yields will vary depending upon a number of factors including species, growth conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
|---|---|---|---|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
| 96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
| Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |