24×10ml Angle Rotor 

Overview

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification

Norgen’s 2X PCR Master Mix is a ready-to-use solution that contains components required for PCR amplification including Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the master mix in order to set up the PCR reaction. This convenient 2X PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of DNA templates with high yields of PCR products.

Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Details

Supporting Data

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Reagents Supplied – Cat # 28007

•  2X PCR Master Mix (1 Vial, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions

Storage Conditions and Product Stability
Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.

Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Methyltetrazine-PEG11-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.

Opentrons Tough 0.2 mL 96-Well PCR Plate, Full Skirt, RNase/DNase-free. Strong polycarbonate frame with thin-walled polypropylene wells for high-performance thermal cycling without warping. Ideal for use within automated liquid handlers with or without a gripper. 0.2 mL total well volume, 0.1 mL working volume. 25 count. Not for therapeutic use SP-2396.