12x10ml PRP Centrifuge Rotor

Overview

• Ensure optimal results for sensitive applications like NGS
• Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
• Purification and enrichment of intact urinary exosomes for functional studies.
• Bind and elute all RNA irrespective of size or GC content, without bias.
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
• No phenol extractions, Proteinase K treatment, nor carrier RNA required.
• No time-consuming ultracentrifugation, filtration nor special syringes are required.
• No precipitation reagents nor overnight incubation required.
• Compatible with urine from any species.
• Pure exosomes are purified and are free-from any other RNA-binding proteins.
• Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.

Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Minimum Urine Input	250 μL
Maximum Urine Input	1 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields	Variable depending on specimen

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

DD6 Floor Standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Application 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Widely used in the area of laboratory, pharmaceutical factory, biochemistry, biological products and so on. It is the ideal instruments for blood separation, protein precipitation and cell collection.

Features 

for DD6 Floor standing 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

1. Brushless DC motor in great torque, free maintenance, no carbon dust pollution, quick in speed up and down.
2. Flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
3. Automatically electric lid lock, super speed, over temperature protection and imbalance protection.
4. Microprocessor control, speed, time, temperature and RCF in operation, speed rising and reducing quick, operate simply.
5.10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
6. There are many rotors for your choice, adapters are available by experiment requirements.
7.3 tiers protection steel cover, safe and reliable.

Technical Parameters 

for 6000rpm 4x1000ml 6x500ml 6x300ml Angle Rotor Laboratory Centrifuge Machine

Max Speed: 6000r/min	Max.RCF: 6600xg
Max Volume: 4x1000ml	Power supply:AC220V,50HZ,10A
Timer: 1~99min	Noise:≤55dBA
Dimension:800×645×830mm	Net Weight: 170KG
Speed Accuracy: ±20r/min	Package(W*D*H): 920×800×1320mm
Temperature range:	Inapplicable

Matched Rotors for DD6 laboratory centrifuge

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No33097	Swing out rotor	4,000	4x1000ml (round/square cup )	4,060
No30221	Angle rotor	6,000	4x300ml	5,390
No30222	Angle rotor	6,000	6x300ml	5,660
No30223	Angle rotor	6,000	6x500ml	6,600

Packing & Shipping_____________________

by Sea/ by Air / by Express Sevices DHL, Fedex, TNT…

DD6 is floor standing large capacity 4x1000ml, 6x300ml, 6x500ml laboratory centrifuge machine without refrigerated one. It is up to max.speed 6000rpm.