Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
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| Kit Specifications | |
| Sample Volume Range | 250 μL |
| Size of RNA Purified | All sizes, including small RNA(<200 nt) |
| Minimum Elution Volume | 50 μL |
| Maximum Elution Volume | 100 μL |
| Time to Complete 10 Purifications | 15 – 20 minutes |
| Average Yield | ≥ 1 μg **Varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Kit Component | Cat. 69100 (50 preps) | Cat. 69300 (192 preps) |
|---|---|---|
| Lysis Buffer A | 30 mL | 100 mL |
| Solution WN | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 20 mL |
| Mini Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Isolation Plate | – | 2 |
| 96-Well Collection Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Product Insert | 1 | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens. A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
K-ISOC
SKU: 700004306
100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Isocitric Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 1 to 80 µg of D-isocitric acid per assay |
| Limit of Detection: | 0.35 mg/L |
| Reaction Time (min): | ~ 3 min |
| Application examples: | Fruit juices, fruit products, soft drinks and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by EN, NEN, NF, DIN, GOST, IFU and AIJN |
The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View our full list of organic acid assay kit products.
Advantages
The D-Isocitric Acid test kit is for the specific and rapid measurement and analysis of D-isocitric acid in fruit juices.