Introduction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate
Applications	PCR, southern bolt and virus detection, etc
Purification method	96 well plate
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column

Principle

This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• High throughput – 96 samples can be processed simultaneously

Kit Contents

Contents	D311701	D311702
Purification Times	1 x 96	4 x 96
HiPure gDNA Plate	1	4
96 well Plate (2.2ml)	1	4
1.6ml Collection Plate	1	4
0.5ml Collection Plate	1	4
Silicon Seal Tape	1	4
Seal Film	5	25
Buffer ATL	30 ml	100 ml
Buffer AL	30 ml	100 ml
Buffer DW1	60 ml	250 ml
Buffer GW2	50 ml	2 x 100 ml
Proteinase K	50 ml	200 ml
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	30 ml	120 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Sample amount	Leukocyte protocol*	Colum type	Elutio volume	Average yield	Time per run
HiPure Blood DNA Mini Kit	D3111	10-200μl	1ml	2ml column	≥20μl	5-9μg/200μl	≤30 minutes
HiPure Tissue&Blood DNA Midi Kit	D3113	0.2-2ml	10ml	1.5ml column	≥300μl	20-40μg/1m	≤80 minutes
HiPure Tissue&Blood DNA Maxi Kit	D3115	3 -10ml	10ml	15ml column	≥700μl	20-40μg/1m	≤90 minutes
HiPure Tissue&Blood DNA 96 Kit	D3117	1-200μl	1ml	96 well plate		3-8μg/200μl

*Note：Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750R MSPU650R or MSPU650 magnet plates and is available as a discounted kit   Picture shown is SKU: MSPU650RKT for reference 

SKU #

MTP96

Features

• SBS/ SLAS footprint
• Laser labeled rows and columns for easy sample control
• Precision manufactured for more consistent results
• Works with strips and individual tubes
• Made in USA 
• For Research use only

Compatibility

• 8-strip 0.2 mL PCR Strips 
• 12-strip 0.2 mL PCR Strips 
• Individual PCR tubes
• PCR Plates (Unskirted-skirt)

The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with

Overview

• Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
• No hazardous chemicals required (e.g. phenol or chloroform)
• Effective lysis with Proteinase K and optional lysozyme treatment
• Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
• Wide compatibility with a variety of food products for GMO-DNA isolation
• Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)

This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.

This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.  

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)	200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Samples Tested by PCR

Food Materials	Samples that Have Been Tested by PCR
Plant related	Cereal, Jam, Chocolate, Spices, Sauce
Animal related	Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product	Milk, Yogurt, Cheese
Pathogens (enriched from food samples)	E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk

Component	Cat. 54500 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer I	7 mL
Buffer SK	30 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K	1 vial
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1