These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 10 µg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (<200 nt) |
| Maximum Blood Input Volume | 3 mL |
| Time to Complete 10 Purifications | 40 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RBC Lysis Buffer should be stored at 4°C upon arrival. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
|---|---|---|---|
| RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
| Buffer RL | 30 mL | 30 mL | – |
| Binding Solution | – | – | 2 x 40 mL |
| Wash Solution A | 38 mL | 38 mL | – |
| Wash Solution | – | – | 2 x 30 mL |
| Elution Solution A | 6 mL | 6 mL | – |
| Elution Solution | – | – | 2 x 20 mL |
| Enzyme Incubation Buffer | – | 6 mL | 1 |
| DNase I | – | 1 Vial | – |
| Mini Spin Columns | 50 | – | – |
| Lysate Homogenization Column | – | 50 | – |
| Single Cell RNA Column | – | 50 | – |
| RBC Lysis 96-Well Plate | – | – | 2 |
| 96-Well Filter Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Collection Tubes | 50 | 100 | – |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 50 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
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