ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
ProbeSure OneStep RT-PCR Master Mix is highly efficient and sensitive, enabling detection of low-abundance RNA targets, particularly useful for applications such as gene expression analysis and viral RNA detection. ProbeSure OneStep RT-PCR Master Mix demonstrates robust performance across a wide range of RNA templates and can be employed for both routine and challenging samples.
ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA. ProbeSure OneStep RT-PCR Master Mix combines reverse transcription (RT) and PCR in a single step. This advanced master mix simplifies workflows by eliminating the need for separate reactions for reverse transcription of RNA to cDNA, and PCR amplification from the newly generated cDNA.
Norgen’s Saliva/Swab RNA Purification Kits provide a rapid method for the purification of total RNA from non-preserved saliva and nasal/throat swabs, and from preserved saliva collected on Norgen’s Saliva RNA Collection and Preservation Devices (Cat. RU53800) or preserved swabs collected in Norgen’s Total Nucleic Acid Preservative Tubes (Cat. 69200). Purification is based on using Norgen’s proprietary resin separation matrix. RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The kit allows the purification of total RNA, including viral and bacterial RNA, irrespective of size or GC content. The purified RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays, and NGS.
This kit offers a range of elution volumes, with a minimum elution volume of 75 μL and a maximum elution volume of 100 μL. Complete 10 purifications in as little as 15 minutes.
High throughput 96-well plate format for rapid purifications with very consistent well-to-well RNA isolation. Purified RNA is suitable for a variety of downstream applications , including Small RNA Sequencing. Find out more information about Norgen’s NGS Services.
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| Kit Specifications | |
| Sample Volume Range | 250 μL |
| Size of RNA Purified | All sizes, including small RNA(<200 nt) |
| Minimum Elution Volume | 50 μL |
| Maximum Elution Volume | 100 μL |
| Time to Complete 10 Purifications | 15 – 20 minutes |
| Average Yield | ≥ 1 μg **Varies from sample to sample |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Kit Component | Cat. 69100 (50 preps) | Cat. 69300 (192 preps) |
|---|---|---|
| Lysis Buffer A | 30 mL | 100 mL |
| Solution WN | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 20 mL |
| Mini Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Isolation Plate | – | 2 |
| 96-Well Collection Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Product Insert | 1 | 1 |
