4x100ml Swing Buckets Rotor

Name of Product

HybriDetect 2T

Catalog Number

MGHD2 1

Short Info

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

5 pg DNA, equivalent to agarose gel electrophoresis

Test Principle

HybriDetect 2T is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for simultaneous detection of two different analytes such as proteins, antibodies or gene amplification products. The results can be interpreted qualitative or quantitative.

The HybriDetect 2T is a simple and quick tool to develop your own rapid test. The 2T is designed for simultaneous detection of two analytes. Various molecules can be detected: Proteins, Antibodies, Genetic amplification products

Product Description

Isothermal Amplification Kit: Freeze-Dried Reagent, Stable & Easy to Operate

Product Detail

 Kit Storage and Term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months 

Isothermal Nucleic Acid Principle Summary

The kit is based on rapid nucleic acid amplification technology at room temperature and constant temperature, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension.

Isothermal Nucleic Acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit Basic
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal Nucleic Acid Applications

Suitable for DNA isothermal rapid amplification kit(Basic type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Colloidal gold probe:Require a sequence of 46-52nt in length

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Introduction

Usages:

For the rapid detection and of coliforms and E. coli.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate were mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the color groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies.

Formulation (per liter):

Peptone 15.0g

Yeast extract powder 3.0g

Sodium chloride: 5.0g

Sodium lauryl sulfate: 0.1g

Agar: 12.0g

Mixed chromogenic substrate: 6.77g

Final pH 7.0 ± 0.2

How to use:
1. Weigh 41.9g of the product, adding , 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into flask, 115 autoclaved 10minutes.

2, Take 25.0g or 25.0mL of sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline) ,shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 medium.

3, observe the results.

Quality control:

This product appears light yellow after  pouring on plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Bacteria name            bacteria NO.       growth situation         feature

Escherichia coli          ATCC25922            good         blue-green colonies

Citrobacter              ATCC8090             good         orange-red colonies

Salmonella typhimurium   CMCC50115          good           colorless colonies

Enterococcus faecalis     ATCC29212           suppressed            —–

Storage: Store in a dark, cool and dry place, tighten the caps immediately after use. Storage period of two years.

1000mL