4x10x7ml Swing Buckets Rotor

Permagen’s MSPU750R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot, and consumable labware inconsistencies.

Also available in a kit form which includes our MTP96 clear top plate for 0.2 mL PCR tubes and strips.   

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

MSP750R (magnet plate only)

MSP750RKT (Includes top plate kit for 0.2 mL strips and tubes)

Features

• SBS/ SLAS footprint
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Beads are pulled to tube walls for easy removal
• Integrated cushion base allowing for accidental contact between tips and well bottoms
• Made in USA 
• For Research use onl

Compatibility

• Full – Skirt PCR plates
• Semi- Skirted PCR plates 
• Non- Skirted PCR plates
• 0.2 mL PCR Strips and tubes  (With kit)
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Minimum – 30 µL 

Maximum – 0.2 mL

Permagen’s MSPU750R is identical to the product shown above, with the exception of the added integrated cushion base which helps aid in robot, and consumable labware inconsistencies.

Overview

Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.

• Heat sealing offers a 100% effective method for plate sealing for a complete seal integrity, as well as being quick and cost effective
• Our Gas PermASeal Heat Seal is made from a non-woven material and is designed for use in cell culture, due to its porous nature
• The seal is compatible with polypropylene and polystyrene
• It can be removed by peeling, or it can be pierced with a pipette tip manually, using a liquid handling robot.
• Gas PermASeal Heat Seal can be utilized for effective overnight incubations, during which it demonstrates significant reductions in evaporation compared to lids
• It can also be used for insect and seed storage as it enables gas exchange, whilst providing an inert surface with no adhesive to interfere with the well contents
• This seal is available as sheets, for use with manual and semi-automated sealers, such as our HeatASeal 500 Sealing Machine
• Also available in multiple roll formats compatible with specified automated heat sealers, such as our Wasp or Chameleon XT

Non-woven gas permeable seal that limits evaporation. The seal is peelable, pierceable; suitable for cell culture and seed & insect storage too.

Overview

• Efficient depletion of bovine exosomes from Fetal Bovine Serum
• Deplete exosome-sized vesicles from versatile FBS volumes
• No protease treatment required
• No time-consuming ultracentrifugation
• No precipitation reagents required
• No overnight incubation required
• Exosome depletion confirmed by reduction of bovine miRNAs below detectable levels
• The depleted FBS provides the same cellular growth rates as the standard FBS
• Purification is based on Norgen’s proprietary Silicon Carbide resin matrix

Norgen’s FBS Exosome Depletion Kits provides a quick and easy protocol for the depletion of bovine exosomes from FBS prior to using it as a growth supplement in your culture medium. The FBS recovered from the depletion process is exosome-depleted and does not contain any quantifiable bovine miRNAs. Moreover, the exosome-depleted FBS will support the growth of your cells of interest similar to the non-depleted FBS. Norgen’s kits allow for the processing of different FBS volumes. The depletion is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require ultracentrifugation, any special instrumentation, precipitation reagents or any protease treatments. More importantly, the depletion process is an inexpensive method for exosome depletion from FBS, as compared to the expensive current ready-to-use exosome-depleted media available on the market.

Background

Most culture medium used for the growth and propagation of cells in culture require the addition of fetal bovine serum (FBS) as a growth supplement to media. FBS is obtained from bovine (cow) serum, and therefore contains large quantities of bovine exosome vesicles. These exosomes may interfere with some types of studies, or may lead to unreliable results when studying the exosomes shed from your cells of interest in normal culture conditions. Therefore, the use of exosome-depleted FBS is highly recommended for many types of studies.

FBS Exosome Depletion Kit (Slurry Format)

For FBS volumes ranging from 140 mL to 280 mL.

FBS Exosome Depletion Kit (Column Format)

For FBS volumes ranging from 120 mL to 240 mL.

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.