This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Specifications
| Features | Specifications |
| Main Functions | IVD5412 precast kit for kingfisher Flex |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technolog |
| Process method | Manual or automatic |
| Sample type | |
| Sample amount | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
Advantages
Kit Contents
| Cat.No | Reagent | IVD5412-F-96 |
| PK/Carrier RNA | 1x 24 mg/Bottle | |
| Protease Dissolve Buffer Blue | 1.8 ml/Bottle | |
| Tip | 1 | |
| Sample Plate (DW Plate) | 500µl Buffer MLB/Well | 1 |
| Wash 1 Plate (DW Plate) | 500µl Buffer MW1/Well | 1 |
| Wash 2 Plate (DW Plate) | 500µl Buffer CW/Well | 1 |
| Beads Plate (DW Plate) | 500µl CW & 20µl MPN/Well | 1 |
| Elute Plate (KF Plate) | 90µl NFW/Well | 1 |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
Usages:
For isolation and cultivation of Listeria monocytogenes.
Principle:
Peptone provide carbon and nitrogen sources; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains osmotic equilibrium; glucose carbon source; agar as medium coagulant.
Formulation(per liter):
Trypsin digest of casein 10.0g
Heart trypsin digest 3.0g
Corn starch 1.0g
Pepsin digests meat 5.0g
Yeast extract powder 8.0g
Sodium chloride 5.0g
Glucose 0.5g
Mannitol 10.0g
Ferric ammonium citrate 0.5g
Esculin 0.8g
Lithium chloride 15.0g
Phenol Red 0.1g
Agar 15.0g
Final pH 7.2 ± 0.2
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
250g
