endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
Total RNA Purification Kit Dx (Spin Column)
This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.
Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)
Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Intended Use
To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.
NOTE: This product is not available for sale in the United States.
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| Kit Specifications | |
| Maximum Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of RNA Purified | All sizes, including small RNA < 200 nt |
| Time to Complete 96 Purifications | 30 minutes |
| Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster) | 15 μg 50 μg 9 μg 26 μg 1 μg |
| Maximum Amount of Starting Material: Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue | 100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. Dx17200 (50 preps) | Cat. Dx24300 (192 preps) | Cat. Dx24350 (192 preps) | Cat. Dx24380 (576 preps) |
|---|---|---|---|---|
| Lysis Solution | 40 mL | – | – | – |
| Buffer RL | – | 2 x 40 mL | 2 x 40 mL | 350 mL |
| Wash Solution | 22 mL | – | – | – |
| Wash Solution A | 22 mL | 2 x 38 mL | 2 x 38 mL | 1 x 148 mL 1 x 74 mL |
| Elution Solution | 6 mL | – | – | – |
| Elution Solution A | – | 2 x 20 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | – | – | – |
| 96-Well Isolation Plate | – | 2 | 2 | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | 6 |
| Collection Tubes | 50 | – | – | – |
| 96-Well Collection Plate | – | 2 | 2 | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | 6 |
| Elution Tubes (1.7 mL) | 50 | – | – | – |
| 96-Well Elution Plate | – | 2 | 2 | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | 6 |
| Adhesive Tape | – | 4 | 4 | 12 |
| Product Insert | 1 | 1 | 1 | 1 |
K-PANOPA
SKU: 700004323
100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Nitrogen, Primary Amino Nitrogen, YAN |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.2 to 10 µg of amino nitrogen per assay |
| Limit of Detection: | 2.59 mg N/L |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Grape juice, must, wine and other materials. |
| Method recognition: | Novel method |
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
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Advantages
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.