This kit provides a rapid spin column method for the isolation and purification of total RNA (including microRNA) from exfoliated cells that have been shed into the urine from the urinary tract. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, as well as from the contaminating species found in urine such as glucose and salts, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real-time RT-PCR, qRT-PCR, Northern blotting, NGS, and expression array assays. Multiple samples can be processed in 20 minutes.
Background
RNA biomarkers from exfoliated cells can be used as non-invasive tools for a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers.
Figure 1 / 1
Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg |
| Volume of Urine Processed | 1-50 mL |
| Maximum Input of Exfoliated Cells | 1 x 106 |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Time to Complete 10 Purifications | 20 minutes (for Exfoliated Cells)30 minutes (for Bacteria) |
| Average Yield | ~1μg RNA per 1 x 105 cells (For Exfoliated Cells – Varies due to cell density sample)~0.5μg RNA per 1 x 107 cells(For Bacteria – Varies due to cell density sample) |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 22550 (25 preps) |
|---|---|
| Buffer SK | 15 mL |
| Wash Solution A | 18 mL |
| Elution Buffer E | 6 mL |
| Micro Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
Techinical parameter
| ltem No | SH7006 | SH9006 | SH9009 |
| Ice condenser capacity | 6kg | 6kg | 9kg |
| Ice condenser temperature | -70℃ | -90℃ | -90℃ |
| Chamber volume | 12L | 15L | |
| Freeze dried chamber | Square cavity/front door opening/door opening angle up to 180 degrees | ||
| Sample shelf | ·Stainless steel template partition● Electric heating/optional | ||
| Vacuum pump | Extreme vacuum5*10mbar ,with a pumping capacity of 8m³/hbuilt-in to the host/optional for various types of pumps | ||
| Oil mist filter | Standard configuration | ||
| Vacuum pump connection pipe | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | ||
| Anti-corrosive treatment | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | ||
| Fxternal valve | 12 ports/optional | 1-48 ports/optiona | |
| Eutectic point detection system | Support/Optional | ||
| Data export analysis | Support/Optional | ||
| Power | 0.95kw | 1.65kw | |
| Size | 700*580*1600mm | ||
Model:
SH7006,SH9006,SH9009
Brand:
Yingtai
t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.
t-Boc-N-amido-PEG4-Amide-Tri-(propargyl-PEG10-ethoxymethyl)-methane is a branched crosslinker molecule with three terminal propargyl groups and a t-Boc protected amide group. The propargyl groups can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The protected amine can be deprotected under acidic conditions. Reagent grade, for research use only.