4x8x15ml Swing Buckets Rotor 

K-AMIARLQ

SKU: 700007403

50 assays (manual) / 500 assays (auto-analyzer)

Content:	50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels

Analyte:	Ammonia, Nitrogen, YAN
Assay Format:	Spectrophotometer, Auto-analyzer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Decrease
Linear Range:	0.4 to 8 µg of ammonia per assay
Limit of Detection:	1 mg/L
Limit of Quantification:	3 mg/L
Reproducibility (%):	< 5%
Reaction Time (min):	~ 5 min

The Ammonia (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of ammonia in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Browse all nitrogen assay kits.

Advantages

• “Ready to use” liquid stable formulation – no reconstitution needed
• Rapid reaction (~ 5 min)
• PVP incorporated to prevent tannin inhibition
• Standard included
• Suitable for manual and auto-analyzer formats
• Quick Reference Guide available
• Mega-Calc™ software tool is available for hassle-free raw data processing

The Ammonia (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of ammonia in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Introduction

Intended Use

For selective enrichment culture of Listeria monocytogenes in food.

Principle and Interpretation

Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; esculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.

Formulation

Ingredients	/liter
Enzymatic digest of animal tissues　( Proteose peptone)	5.0g
Enzymatic digest of casein　( Tryptone)	5.0g
Meat extract	5.0g
Yeast extract	5.0g
Sodium chloride	20.0g
Disodium hydrogen phosphate dihydrate	12.0g
Potassium dihydrogen phosphate	1.35g
Aesculin	1.0g
Lithium chloride	3.0g
pH7.2±0.2 at 25°C

Preparation

Suspend 57.4g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃,aseptically add the contents of 1 vial of Fraser Selective Supplement (SR0120) to each 225 mL of base to prepare FB1 solution; Add one reagent (SR0130) to each100 mL of FB2 culture medium to prepare FB2 enrichment solution.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 46~50 hours

Quality control strains	Growth	Characteristics
Listeria monocytogenes ATCC19115 + Escherichia coli ATCC25922 + Enterococcus faecalis ATCC29212	>20 cfu  On PALCAM	Gray to black colony count with black halo
Escherichia coli ATCC25922	< 100 colonies on TSA	Inhibition
Enterococcus faecalis ATCC29212

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Revision

On June 14, 2024

References

ISO 11290

Intended Use For selective enrichment culture of Listeria monocytogenes in food. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen ……