TXK4 12Cards Rotor

Introduction

029110 Sterile Liquid Paraffin

Usage:For covering a liquid medium for anaerobic culture.

Specification:10ml.

10ml

Overview

• Removes primer dimers
• Simultaneously clean-up and normalize PCR products 
• Fast (less than 20 minutes), high-throughput and easy processing using centrifugation
• Sufficient elution volume (100 µL) for repeat or future assays 
• Non-magnetic bead purification 

Norgen’s NGS Normalization 96-Well Kit allows for high-throughput NGS library PCR amplicon clean-up and normalization of NGS library PCR product concentration. The clean-up removes all PCR by-products including primers, dimers, enzymes and unincorporated nucleotides during the library prep PCR step. Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. The amount of Norgen’s matrix in each 96 well is optimized to have a limited binding capacity, thus each well can elute an equal concentration of PCR product. The process involves first adding 3 volumes of Buffer SK to the PCR product, and the mixture is then loaded onto the 96-Well Normalization Plate. The bound PCR amplicon is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified PCR amplicon is eluted with Elution Buffer B. The purified and normalized library PCR amplicon can then be used in NGS workflows and other sequencing applications.

Details

Supporting Data

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Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 61900 (192 preps)
Buffer SK	30 mL
Wash Solution A	2 x 38 mL
Elution Buffer B	30 mL
96-Well Normalization Plate	2
96-Well Collection Plate	2
96-Well Elution Plate	2
Adhesive Tape	1
Product Insert	1

Description

Specifications:

Clone	IHC512
Source	Mouse Monoclonal
Positive Control	Hairy Cell Leukemia
Dilution Range	1:200

Annexin A1 (ANXA1) is a membrane protein that plays a role in innate and adaptive immunity by controlling the biosynthesis of inflammation, prostaglandins, and leukotriene mediators. This target is overexpressed in 97% of all samples from patients with with hairy cell leukemia, and is absent in other B-cell lymphomas. High ANXA1 expression is frequently associated with advanced stage esophageal and esophagogastric junction adenocarcinoma, and is also linked to advanced and metastatic disease states.