Propargyl-PEG3-alcohol consists of a hydroxyl group and a propargyl group. The propargyl group reacts with azide compounds in copper catalyzed azide-alkyne Click Chemistry to form a triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG3-alcohol consists of a hydroxyl group and a propargyl group. The propargyl group reacts with azide compounds in copper catalyzed azide-alkyne Click Chemistry to form a triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.
Free Download
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
Figure 1 / 3
Click for expanded view
| Kit Specifications | |
| Maximum Stool Input | 200 mg (fresh/frozen stool) or 400 μL (preserved stool) |
| Type of Stool Processed | Preserved, fresh, and frozen stool from humans and animals |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 600 μL |
| Time to Complete 10 Purifications | 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 49500 (50 preps) |
|---|---|
| Lysis Buffer C | 60 mL |
| Wash Solution A | 38 mL |
| Elution Buffer E | 6 mL |
| Bead Tubes | 50 |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
