Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
RLE500
Maximum – 150 µL
Minimum – 5 µL
Designed for those labs wishing to separate magnetic beads into rings.
The Permagen ring magnet low elution plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate |
| Applications | Cell transfection, animal injection, etc. |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Low copy plasmid vector |
| Sample amount | 1-5ml LB（x96） |
| Yield | 30μg |
| Elution volume | ≥75μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 70μg |
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once;
2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit;
3. Detoxification: endotoxin content <0.1EU/μg;
4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
| Contents | P115701 | P115702 | P115703 |
| Purification Times | 1 x 96 Preps | 4 x 96 Preps | 20 x 96 Preps |
| RNase A | 10 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 550 ml |
| Buffer P2 | 30 ml | 120 ml | 550 ml |
| Buffer LEN3 | 15 ml | 60 ml | 300 ml |
| Buffer LN4 | 80 ml | 270 ml | 2 x 700 ml |
| Buffer LN5 | 40 ml | 220 ml | 1100 ml |
| Buffer PW1 | 40 ml | 220 ml | 1100 ml |
| Buffer PW2 | 25 ml | 100 ml | 2 x 200 ml |
| Elution Buffer | 30 ml | 60 ml | 250 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 1.6ml Collection Plate | 2 | 8 | 40 |
| 0.5ml Collection Plate | 1 | 4 | 20 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
For any technical problems or customized products, please contact us.
F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
DBCO-STP Ester is an amine-reactive, water-soluble labeling reagent, including a DBCO group for click reactions with azide groups on target molecules and an STP ester group, which can react with primary amines, forming covalent amide bonds. STP esters typically display much better stability toward hydrolysis in aqueous media, resulting in more efficiency and better reproducible labeling of biopolymers. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-STP Ester is an amine-reactive, water-soluble labeling reagent, including a DBCO group for click reactions with azide groups on target molecules and an STP ester group, which can react with primary amines, forming covalent amide bonds. STP esters typically display much better stability toward hydrolysis in aqueous media, resulting in more efficiency and better reproducible labeling of biopolymers. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.