PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
PEG3-(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)-(Amino-Tri-(carboxyethoxymethyl)-methane) is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid groups can react with primary amino groups in the presence of activators (e.g. HATU) to form a stable amide bond.
| Clone | IHC626 |
| Source | Mouse Monoclonal |
| Positive Control | Stomach |
| Dilution Range | 1:200 |
Mucin 6 (MUC6) is a glycoprotein expressed in mucous neck cells, pyloric glands of the antrum, epigastric and bronchial epithelium, and in Müller ducts of the endocervix and urethral epithelium. Anti-MUC6 is useful for differentiating fetal, precancerous, and cancerous colonic mucosa from normal colon, as the antibody does not stain the latter. Anti-MUC6 stains the gastric epithelial surface of normal human gastrointestinal tract.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol |
| Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (vacuum) |
| Sample type | Serum, plasma and other cell-free fluid samples |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.
| Contents | IVD3182 |
| Purification Times | 50 |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Protease Dissolve Buffer | 30 ml |
| Carrier RNA | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml Collection Tubes | 100 |
| Extender Tube | 50 |
| Vac-Connector | 50 |
Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Experiment Data
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,