Product Details

Sample Type:

Serum, Plasma, Whole Blood

Package Size:

48 Tests/Kit

Test Time:

5-20 Minutes

Target Disease:

African Swine Fever Virus

Product Name:

African Swine Fever Virus Isothermal Detetction Fluorescence Kit

Test Principle:

Nucleic Acid Detection

Target Species:

Pigs

High Light:

Livestock porcine detection kit

, 

porcine detection kit 48 Tests/Kit

, 

5-20 Minutes porcine test kit

Product Description

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection 

Product parameters:

Kit composition (WLRE8208KIT 48T/Pack)
Composition	Content
E buffer	1 mL×2 Tubes
B buffer	150 μL×1Tube
Positive control template	100 μL×1Tube
Reagents	48T

Product features and advantages:

Product features and advantages
Features	Advantages
High sensitivity	1 copies/ml
Strong specificity	Specificity is superior to PCR
Short reaction time	20min
Polymorphic reagent	Liquid, freeze-dried powder, freeze-dried ball
Easy to operate	Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport	It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment	Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector

Product application:

Application
Ultra-clean laboratory	African Swine Fever Virus Isothermal Detection
Indoor

Operation procedure and process:

Operation procedure and process:

Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.

Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.

Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid

Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control

Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)

Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.

Performence Comparison Data:

No.	TT value	Template concentration	Result determination
1	4.2	10-1	Positive
2	5.5	10-2	Positive
3	10.2	10-3	Positive
4	17.0	10-4	Positive
5	12.0	10-5	Positive
6	–	10-6	Negative
7	–	10-7	Negative
8	Negative control		Negative

Support and Services:

Livestock Disease Kit Technical Support and Services

We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.

• Our technical support staff is available to answer any questions you may have about the product.
• We offer online tutorials and user guides to help you understand the product better.
• We provide technical assistance to help troubleshoot any issues you may be having with the product.
• We offer a warranty on our products.

If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.

FAQ:

• Q: What diseases can Livestock Disease Kit detect?
  A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
• Q: How does Livestock Disease Kit work?
  A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
• Q: How long does it take to get results from Livestock Disease Kit?
  A: Livestock Disease Kit can give a result within 5-20 mins.
• Q: What type of sample is suitable for Livestock Disease Kit?
  A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
• Q: Does Livestock Disease Kit require professional equipment?
  A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine

Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection

Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG14 is a homobifunctional crosslinker that can participate in Click Chemistry to yield a stable triazole linkage with azide; copper is needed as a catalyst. With PEG chain embedded in the molecule, the hydrophilicity is greatly improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.

Compared to other domestic products, Magen was the first to solve the stability problem of the column. Many other brands have unstable extraction concentrations, and the longer the time, the more unstable the column becomes. However, in our test of Magen kit, the quality and yield of plasmid extraction still remain stable after 5 years’ storage. For different customers, our kits can be customized. For example, Classic type is suitable for customers with low copy or unclear plasmid types. The rapid type is suitable for customers with high copy plasmids. Compared to many other brands, the plasmid DNA extracted by Magen has a longer preservation time and more thoroughly RNA removal.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 35μg plasmid DNA from 1-5ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	High copy plasmid: 1-5ml culture mediumLow copy number plasmid : 5-10ml culture medium
Yield	5-35µg
Elution volume	≥30μl
Time per run	Complete 1-24 samples in 30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield – up to 35µg plasmid can be binded in one column

Kit Contents

Contents	P100102	P100103
Purification Times	100 Preps	250 Preps
RNase A	5 mg	10 mg
Buffer P1	30 ml	80 ml
Buffer P2	30 ml	80 ml
Buffer P3	40 ml	100 ml
Buffer PW1	60 ml	140 ml
Buffer PW2*	20 ml	50 ml
Elution Buffer	15 ml	30  ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data

Purchase Guide

Name	CAT NO	Features	Sampleamount	Elutionvolume	Yield
HiPure Plasmid Mini Kit	P1001	Classic, Regular application	1-5ml LB	30-50μl	5–35µg/5ml
HiPure Plasmid Mini Kit II	P1002	Classic, Regular application	5-15ml LB	75-100μl	20-80µg/15ml
HiPure Plasmid Plus 96 Kit	P1006	Classic, Regular application	1-1.5ml LB	70-150μl	1-15μg/1ml
HiPure Fastfilter Plasmid Midi Kit	P1013	Fast	30-50ml LB	0.3-0.8ml	50–250µg/50ml
HiPure Fastfilter Plasmid Maxi Kit	P1014	Fast	100-200ml LB	0.5-1.5ml	0.4–1mg/200ml
Low endotoxin/Endotoxin-free Plasmid
HiPure Plasmid EF Maxi Kit	P1156	Classic method, General for all kinds of vectors	100-200ml LB	≥0.7ml	200–1500µg
HiPure Plasmid EF Mini Kit	P1154	Recommend for low copy vector, Thoroughly remove RNA	5-15ml LB	≥60μl	10–80µg
HiPure Plasmid EF 96 Kit	P1157	Recommend for low copy vector, Thoroughly remove RNA	1-5ml LBx96	≥75μl	30µg
HiPure Plasmid EF Midi Kit	P1231	Recommend for High copy vector, Low elution volume, High concentration	25-50ml LB	≥100μl	10-500μg
HiPure Plasmid EF Mega Kit	P1116	Recommend for High copy vector(>3μg/ml)	500ml LB	≥2ml	1-10mg

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.