4x100ml Angle Rotor 

TG16E Technical Parameter：

Max. Speed	16000rpm
Max. RCF	19040×g
Max. Capacity	6×10ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Voltage(V/Hz)	AC 220V/110V  50HZ/60HZ
Size (W x D x Hmm)	370×290×215mm
Net Weight(Kg)	16KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TG16E

Order No	Rotor	Max speed (rpm)	Max Volume(ml)	Max RCF (g)
16E-1	Angle rotor	14000	4×8PCR	12070
16E-2	Angle Rotor	16000	12×1.5/2ml	17940
16E-3	Angle Rotor	14000	24×1.5/2ml	17950
16E-4	Angle Rotor	16000	10×5ml	17880
16E-5	Angle Rotor	13000	6×10ml	14190
16E-6	Angle Rotor	12000	24 pieces capillary vessel	15800
16E-7	Angle Rotor	16000	40×0.2ml	19040
16E-8	Angle Rotor	13000	40×0.5ml	17210
16E-9	Angle Rotor	13000	30×0.5ml	13900

Features:
1. Brushless motor, no pollution, free-maintenance.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting, operate simply.
3. Electric lid lock, super speed, imbalance protection. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
4. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
5. 3 tiers protection steel cover and get the ideal centrifugation result

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70200 (24 preps)	Cat. 70210, 70220, 70230, 70240 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V1-V3 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

For the detection of the SARS-CoV-2 variants with the VUI-21APR-01 and VUI-21APR-03 (India)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix