genesig® kits are sold for research use only and are not licensed for diagnostic procedures.
Exceptional value for money
Rapid detection of 2019-nCoV
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
These kits concentrate urine proteins while simultaneously removing salts, urea, and other urine contaminants. There is no molecular weight cut-off and therefore the columns capture total urinary proteins and peptides of all sizes making them ideal for biomarker discovery work, differential expression of proteins in various diseases, or other diagnostic research. The columns are convenient, rapid and easy to use and thus offer significant time savings over classic dialysis protocols. The resulting high-quality protein sample is concentrated and free from the original sample salts, thus preparing the sample conveniently for downstream proteomic applications including SDS-PAGE, 2D Gels, MALDI-TOF, LC/MS, LC/MS/MS, whole protein mass spectrometry, western blotting, protein microarrays, and more.
ProteoSpin™ Urine Protein Concentration Micro Kit
Each spin column is able to concentrate and desalt up to 200 μg of urine proteins. Twelve samples can be processed in 20 minutes.
ProteoSpin™ Urine Protein Concentration Midi Kit
The ProteoSpin™ Urine Protein Concentration Midi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 1 to 5 mL inputs of urine. Each mini spin column is able to concentrate and desalt up to 3 mg of urine proteins in 30 minutes.
ProteoSpin™ Urine Protein Concentration Maxi Kit
The ProteoSpin™ Urine Protein Concentration Maxi Kit provides a fast and simple procedure for concentrating dilute solutions of urine proteins from 2 to 20 mL inputs of urine. Each maxi spin column is able to concentrate and desalt up to 4 mg of urine proteins in 45 minutes.
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Kit Specifications | |
Maximum Urine Input Volume | 20 mL |
Maximum Recovered Protein | 4 mg |
Minimum Elution Volume | 2 mL |
Time to Process 4 Samples | 45 minutes |
Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Component | Cat. 17400 (25 preps) | Cat. 52300 (10 preps) | Cat. 21600 (4 preps) |
---|---|---|---|
Wash Solution C | 60 mL | 60 mL | 130 mL |
Binding Buffer A | 4 mL | 4 mL | 8 mL |
Elution Buffer C | 8 mL | 30 mL | 30 mL |
Protein Neutralizer | 4 mL | 4 mL | 4 mL |
Micro Spin Columns | 25 | – | – |
Midi Spin Columns (assembled with collection tubes) | – | 10 | – |
Maxi Spin Columns (assembled with collection tubes) | – | – | 4 |
Collection Tubes | 25 | – | – |
Elution Tubes (1.7 mL) | 25 | – | – |
Midi Elution Tubes | – | 10 | – |
Elution Tubes (50 mL) | – | – | 4 |
Product Insert | 1 | 1 |
Clone | IHC615 |
Source | Mouse Monoclonal |
Positive Control | Tonsil, Follicular Lymphoma, Diffuse Large B-Cell Lymphoma |
Dilution Range | 1:200 |
LMO2, also known as LIM-Only transcription factor 2, RBTN2, or TTG2, is an oncoprotein that is expressed in normal germinal center B-cells, as well as bone marrow hematopoietic precursors and endothelial cells. LMO2 plays a role in angiogenesis and hematopoesis, and its expression has been detected in erythroid and myeloid precursors, megakaryocytes, and also in lymphoblastic and acute myeloid leukemias. LMO2 protein expression has been noted in diffuse large B-cell lymphoma, the most common adult non-Hodgkin lymphoma, as well as follicular lymphoma, a neoplasm derived from germinal center B-cells that accounts for a number of cases of non-Hodgkin lymphomas.
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