MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Specifications
| Features | Specifications |
| Main Functions | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
| Applications | Sequencing, gene chip and qPCR, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | Appropriate |
| Elution volume | ≥15μl |
| Operation time | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excessprimes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
Kit Contents
| Contents | BXP-5 | BXP-50 | BXP-500 |
| MagPure A3 | 5 ml | 50 ml | 500 ml |
Storage and Stability
MagPure A3 should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.DO NOT FREEZE.
MagPure A3 utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
endo-BCN-PEG2-NHS ester is aclick chemistry crosslinker containing an NHS ester group. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group is reactive with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
endo-BCN-PEG2-NHS ester is aclick chemistry crosslinker containing an NHS ester group. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group is reactive with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
