Introduction

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA and miRNA from cell and tissue without MagZol reagent
Applications	RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cell, Animal tissue, Plant tissue
Sample amount	Cells: ≤ 5 x 10^6, Animal tissue:<10mg
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

Advantages

• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – several samples can be extracted in 40 minutes
• High applicability – samples including animals, plants, bacteria, cells, etc.
• High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Safe – no phenol chloroform extraction and no use of Trizol reagent

Kit Contents

Contents	R431102	R431103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	100	2 x 250
2ml Collection Tubes	100	2 x 250
Proteinase K	48 mg	240 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer RLC	40 ml	200 ml
Buffer RWC	20 ml	80 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

Product Description

Long-Term Stable Nucleic Acid Reagent For RNA Extraction

Product Description:

Amp-future Bio’s Nucleic Acid Extraction Reagent is a RNA extraction kit that provides the highest quality nucleic acid extraction. The RNA extraction kit is an automated nucleic acid extractor which allows for quick and easy RNA isolation. The reagent is in liquid form and has a shelf life of 12 months, making it a reliable and durable product. With the Nucleic Acid Extraction Reagent, Amp-future Bio provides the accuracy and reliability of a professionally designed RNA extraction kit.

Features:

• Product Name: RNA Nucleic Acid Extraction Reagent
• Brand: Amp-future Bio
• Form: Liquid
• Shelf Life: 12 Months
• Application: RNA Extract
• Keywords:  RNA Extraction Kit 

Technical Parameters:

Property	Value
Form	Liquid
Product Name	RNA Nucleic Acid Extraction Reagent
Application	RNA Extraction
Brand	Amp-future Bio
Shelf Life	12 Months
Composition	5ml*4 tubes

Applications:

Amp-future Bio’s Nucleic Acid Reagent is an advanced automated nucleic acid extraction solution designed to efficiently extract RNA from biological samples while maintaining quality and accuracy. It is made in China and has a 12-month shelf life. It comes in a liquid form and is suitable for use in automated nucleic acid extractors, allowing for automatic and highly efficient extraction of nucleic acid from various sample types. The reagent is also designed to be easy to use, making it an ideal solution for laboratories that require fast and reliable results. This reagent is a great choice for any research or clinical laboratory that needs efficient and accurate nucleic acid extraction.

Support and Services:

Technical Support and Services for Nucleic Acid Reagent
We provide technical support and services for Nucleic Acid Reagents. Our experts are available to help you identify the right reagents for your application, answer any questions, and provide assistance on the use and optimization of these reagents. Our experts are also available to provide advice on troubleshooting and protocol optimization. We offer a range of services to make ordering and stocking of reagents easy and convenient.

We provide:

• Expert advice on the selection of the right reagents for your application
• Free samples for evaluation
• Bulk and custom orders
• Technical support and troubleshooting assistance
• Secure and reliable online ordering
• Fast and convenient shipping options

Please contact us for more information about our technical support and services for nucleic acid reagents.