24×1.5/2ml Angle rotor 

Name of Product

HybriDetect Cassette

Catalog Number

MGHC 1

Short Info

The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

10 pmol/LFA – 1 fmol/LFA

Test Principle

Milenia HybriDetect Cassette is a ready-to-use, universal test unit, which is based on the lateral flow technology using gold nanoparticles. The test unit is designed to develop qualitative or semi-quantitative rapid test systems for several analytes such as proteins, antibodies, or gene amplicons. The user needs to develop an analyte-specific solution or reaction (PCR, RPA, LAMP, CRISPR/Cas, …), which contains a first detector (e.g. antibody, antigen, specific probe) labelled with FITC or FAM and a second one (e.g. antibody, primer) labelled with biotin.

The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.

The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.

With library multiplexing, unique index sequence is added to individual sample during NGS library preparation.  Therefore, each DNA molecule can be identified after pooling of multiple samples based on the index information they have.

Each of our index primers contains a unique index sequence with 6 bases that can be used to identify libraries. Library multiplexing up to 48 samples is possible.

Multiplexing Index Primers (illumina platform): Even distribution of 48 samples using index primers. 48 libraries were made using the BioDynami NGS DNA Library Prep Kit (Cat. # 30009) and the BioDynami Multiplexing Index Primers (Cat. # 30072). Libraries were pooled at equal concentration and sequenced on the illumina HiSeq 2500. The numbers of reads from 48 libraries were analyzed.

List of index sequence for the primers (each of the index primer mix contains universal primer and one of the index primers). Index number and the index sequence are listed.

List of indexes can be downloaded Here.

Sequence of the final library with index location:

The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.

Overview

• Detection kits for Epstein-Barr Virus (EBV)
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s EBV TaqMan PCR Lyophilized Kit is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s EBV TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of EBV specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM41050L (100 preps)	Cat. TM41010L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
EBV Primer & Probe Mix (Lyo)	280 μL	280 μL
EBV Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1