Norgen’s Plant/Fungi Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plants. Total RNA can be purified from fresh or frozen plant tissues, plant cells or filamentous fungi samples using this kit. All sizes of RNA are purified, including microRNA (miRNA) . The procedure is rapid and convenient.
The RNA is purified without the use of phenol or chloroform. The purified RNA is of the highest quality, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Norgen’s Plant/Fungi Total RNA Purification Kit is also available in a 96-well (High Throughput) format for high throughput applications. Purification with the 96-well plates can be performed using either a vacuum manifold or centrifugation.
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| Kit Specifications – Spin Column | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material: Plant Tissues Plant Cells Fungi | 50 mg 1 x 106 cells 50 mg (wet weight) |
| Average Yield* 50 mg Tomato Leaves 50 mg Tobacco Leaves 50 mg Plum Leaves 50 mg Grape Leaves 50 mg Peach Leaves | 60 μg 60 μg 32 μg 35 μg 30 μg |
| Time to Complete 10 Purifications | 30 minutes |
* Yield will vary depending on the type of sample processed.
* Yield will vary depending on the type of sample processed.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Tobacco (Nicotiana tabacum)
Tomato (Lycopersicon esculentum)
Pepper (Capsicum annuum)
Potato (Solanum tuberosum)
Arabidopsis thaliana1
Peach (Prunus persica)
Apple (Malus sp.)
Pear (Pyrus sp.)
Grape vine (Vitis sp.)
Plum (Prunus sp.)
Palm (Arecaceae)
Pine needle (Pinaceae)
Strawberry
Raspberry
Blackberry
Herbs
Persimmon (Ebenaceae)
Potato tuber (Solanum)
Plum fruit
Citrus
Vanilla bean
Cotton (Gossypium)
Mangrove
Chrysanthemum
Grape berry skin
Kiwi leaves
Peach (fruits and flowers)
Soy bean (legume)
Eastern White Red Cedar
Corn leaves
Cucumber leaves
Aspergillus niger
Mucor racemosus
Cladosporium cladosporioides
Fusarium oxysporum
Penicillium sp.
Botrytis cinerea (Botryotinia fuckeliana)
Pichia sp.
Rhizopus oryzae
Alternaria tenuissima
| Component | Cat. 25800 (50 preps) | Cat. 31350 (100 preps) | Cat. 25850 (250 preps) | Cat. 31900 (192 preps) |
|---|---|---|---|---|
| Lysis Buffer C | 60 mL | 1 x 30 mL, 1 x 60 mL | 3 x 60 mL | 2 x 60 mL |
| Wash Solution A | 38 mL | 38 mL | 1 x 18 mL, 2 x 38 mL | 2 x 38 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL | 20 mL |
| Filter Columns | 50 | 100 | 250 | – |
| Spin Columns | 50 | 100 | 250 | – |
| 96-Well Plate | – | – | – | 2 |
| Adhesive Tape | – | – | – | 4 |
| Collection Tubes | 100 | 200 | 500 | – |
| 96-Well Collection Plate | – | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | – |
| 96-Well Elution Plate | – | – | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 |
SKU: CB024
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Permagen’s SBS/ANSI footprint 24-Tube thermal block allows the use of up to twenty four 1.5 mL -2 mL Microcentrifuge tubes, or screw-cap vials for keeping sample temperatures consistent. Can be used for heating as well
Spring Viremia of Carp is caused by Rhabdovirus carpio, a bullet-shaped RNA virus. The disease has been reported in common carp (or koi) (Cyprinus carpio), grass carp (Ctenopharyngodon idella), bighead carp (Aristichthys nobilis), silver carp (Hypophthalmichthys molitrix), and Crucian carp (Carassius carassius), a close relative of the goldfish. Recent evidence suggests that common goldfish (C. auratus) are also susceptible.
The disease was initially diagnosed in Yugoslavia (Fijan et al. 1971). Since then, it has been identified in other European countries, Russia, and the Middle East. Mortality has reached 70% in yearling carp from European populations. Adult fish can also be affected but to a lesser degree.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings