The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
The kit contains HL-dsDNase which efficiently removes gDNA from RNA preps before running RT-qPCR.
The high activity of the HL-dsDNase at low temperature and its heat-lability makes it ideal to use in a combination with a heat activated RT enzyme. The contaminating genomic DNA is cleaved at ambient temperature and increasing the temperature over 50°C will inactivate the DNase and start the Reverse Transcriptase.
Do you require gDNA removal in applications other than RT-qPCR? Contact our support team for assistance in implementing dsDNase treatment in your workflow.
The Heat&Run® gDNA removal kit removes contaminating gDNA from RNA prior to RT-qPCR.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at low temperatures (5 minutes at 58ºC or 15 minutes at 55ºC).
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Extract viral RNA/DNA from 200μl plasma/serum samples |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant |
| Sample amount | 200μl |
| Yield | 2-10μg |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Kit Contents
| Contents | IVD4173 |
| Purification Times | 100 Preps |
| HiPure Viral Mini Column | 100 |
| 2ml Collection Tubes | 200 |
| PK/Carrier RNA | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AL | 30 ml |
| Buffer MW1 | 44 ml |
| Buffer MW2 | 50 ml |
| RNase Free Water | 15 ml |
Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12 months.
Experiment Data
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
K-MALTA
SKU: 700004315
100 assays (50 of each) per kit
| Content: | 100 assays (50 of each) per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase, β-Amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.05 U/mL |
| Reaction Time (min): | ~ 20 min (Ceralpha Method), ~ 10 min (Betamyl-3 Method) |
| Application examples: | Cereal flours, malts, fermentation broths and other materials. |
| Method recognition: | “Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method |
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
Other enzyme activity test kits available.
Advantages
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.