DNA Concentrator (Magnetic Beads)
The DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration without the need of a DNA vacuum concentrator. Solid Phase Reversible Immobilization (SPRI) magnetic beads are well used for DNA and RNA purification and concentration. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to DNA and RNA.
The concentrator protocol is simple: mix Concentrator Buffer and Concentrator Beads with the sample, wash, and elute pure DNA in a small volume to concentrate the DNA samples. Moreover, the DNA/RNA samples are also purified during the procedure. The beads with our unique technology purify DNA/RNA samples effectively by removing unwanted components such as dNTPs, enzymes, detergents, proteins, and other contaminants.
However, traditional magnetic beads can only bind nucleic acids that are 100 bp or longer. Nucleic acids shorter than 100 bp are not effectively recovered. We have successfully developed the kit that overcomes the hurdle of short nucleic acids recovery. The reagent can be used for concentration of samples from genomic DNA to short DNA/RNA.
Recovery rate
Features
The DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration without the need of a DNA vacuum concentrator. Solid Phase Reversible Immobilization (SPRI) magnetic beads are well used for DNA and RNA purification and concentration. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to DNA and RNA.
Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.
Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.
Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.
| Component | Cat. 25710 (50 rxns) | Cat. 25720 (200 rxns) |
|---|---|---|
| DNase I | 1 vial / 1,600 units | 4 vials (1,600 units/vial) |
| Buffer DR | 1 mL | 4 x 1 mL |
| Enzyme Incubation Buffer | 6 mL | 4 x 6 mL |
| Product Insert | 1 | 1 |
