12×15ml Angle Rotor（Plastic） 

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)

Reverse Transcription, target specific primers (RNA genome viruses only)

Copy number standard curve (sufficient for multiple standard curves)

Internal extraction control – Read through VIC channel*

Endogenous control (150 tests)

RNAse/DNAse free water

*alternative fluorophores available on request

Description 

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 10⁸–10⁹ cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Features

• Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation. 
• Fast and Easy– only few steps for preparation; suitable for time-saving transformation 
• High efficiency– up to 109 cfu/μg 
• Personalization– suitable for most E. coli strains

Kit Contents

					Component	Volume
					Champion™ CC Buffer	20 ml
					SMO-Broth™	100 ml x 2
					pUC19 Control Plasmid (10-4 μg/μl)	5 µl
					Instruction Manual	1
					Champion™ Competent Cell Preparation Card	1

Storage

4°C for 12 months

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Overview

• The preservative provides sample homogeneity eliminating sample variability
• Preserve and transport DNA & RNA safely at ambient temperature
• Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
• No cold shipping/storage needed – hassle-free and cost effective
• Isolate nucleic acids for any application including 16s NGS
• Robust preservation over a range of temperatures
• Customizable with various accessories for easy and safe collection
• Eliminates odor and renders samples safe and non-infectious

Norgen’s Stool Nucleic Acid Collection and Preservation Tubes are designed for the rapid preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Tubes contain Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Stool Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Stool Preservative allow samples to be stored at room temperature for over 2 years for DNA and 7 days for RNA. To extend the stool RNA stability in the preservative, storage at -20°C or -70°C is recommended at the arrival to testing facilities until the RNA purification.

Free Download

Extracting Biological Insights from Stool

Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free

Details

Supporting Data

Figure 1 / 7

Previous

Next

Click for expanded view

Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will vary depending on the samples

Storage Conditions and Product Stability

All tubes should be kept tightly sealed and stored at room temperature (15 – 25 °C) for 2 years after the date of shipment without any reduction in performance.

Kit Components	Cat. 45660
Stool Nucleic Acid Collection and Preservation Tubes	50
Product Insert	1