Overview
Norgen’s Nuclease-Free Water is free of all DNases, RNases and nucleic acids and is suitable for all molecular biology applications requiring nuclease-free water including PCR, RT-PCR and real-time PCR. Our water is prepared without the use of DEPC, and is ready to use without further treatment.
Available Sizes
Applications
For use in any molecular biology application
Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.
Precautions and Disclaimers
This product is designed for research purposes only. It is not intended for human or diagnostic use.
Optically clear adhesive film with pressure activated adhesive. Seal is peelable; suitable for qPCR and other imaging techniques including crystallization.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Rapidly Extract viral DNA/RNA from 200μl non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, etc. |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral DNA / RNA, body cell DNA / RNA |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Plasma, serum, effusion, urine, fecal suspension supernatant |
| Sample amount | 200μl |
| Elution volume | ≥30μl |
| Time per run | |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 MmTris, pH 8.0).
Advantages
Kit Contents
| Contents | IVD4175 |
| Purification Times | 100 Preps |
| HiPure Viral Column | 100 |
| 2ml Collection Tubes | 100 |
| PK/Carrier RNA | 50 mg/310μg |
| Protease Dissolve Buffer | 5 ml |
| Buffer VLE | 42 ml |
| Buffer CE | 60 ml |
| RNase Free Water | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.