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K-ACETAF
SKU: 700004255
141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL)
| Content: | 141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Acetic Acid |
| Assay Format: | Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | up to 30 μg/mL of acetic acid per assay |
| Limit of Detection: | 10 mg/L (recommended assay format) |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by EN, ISO, ICUMSA, IFU and MEBAK |
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products.
See more of our acetic acid and organic acid test kits.
Advantages
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.
Specifications
| Features | Specifications |
| Main Functions | Isolation bacterial DNA from cultures, food and other samples |
| Applications | PCR, southern blot and enzyme digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Culture medium, swab, parasitic blood, tissue,sputum, etc. |
| Sample amount | Bacterial culture medium: 0.5-2 mlTissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.
Kit Contents
| Contents | D314602 | D314603 |
| Purification Times | 50 Preps | 250 Preps |
| Hipure DNA Mini Columns I | 50 | 2 x 125 |
| 2ml Collection Tubes | 50 | 2 x 125 |
| Glass Beads (0.1~0.2mm) | 20 g | 100 g |
| Buffer P1 | 20 ml | 100 ml |
| Buffer DL | 15 ml | 80 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Lysozyme | 60 mg | 300 mg |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
Storage and Stability
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.