Description
The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM2700 ExcelBand™ 3-color Broad Range Protein Marker resolves 13 major bands in SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2700 ExcelBand™ 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Control System:
5.4”LCD Touch Screen Display
>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.
>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.
>Ithas the functions of cold trap precooling and vacuum pump preheating,and the time can be set by the userin the system. >Display whether the current running status is normal or not by numerical color.
>Built-in saturated vapor pressure &temperature corresponding relationship table,dearly displaying the corresponding relationship.
o One-key operation,the operation is extremely simple,no special professional learning and training is required.
o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably.
o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.
o Ambient temperature monitoring,the device automatically reminds the alarm if the ambient temperature is too high.
o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.
Drying chamber | · The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional) |
Cold trap andcondensing coil | · The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation. |
Sample shelves | · 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf. |
Refrigerationsystem | · Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures. |
Techinical parameter
| Item No | ST7003 | ST7006 | ST9006 | |
| Ice condenser capacity | 3kg | 6kg | 6kg | |
| Ice condenser temperature | -70℃ | -70℃ | -90℃ | |
| Cold trap volume | 5L | 10L | 10L | |
| Freeze dried chamber | Bell shape (standard configuration)/Multi manifold type/T-shaped frame type | |||
| Sample shelf | φ200mm.Single layer area 0.03m2 with 3-5 layers available | ●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona | ||
| Vacuum pump | Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types | |||
| Oil mist filte | Standard configuration | |||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | |||
| Anti-corrosive treatment | Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents. | |||
| External valve | 6pieces/optional | 1-48 pieces/optional | ||
| Eutectic point detection system | Support/Optional | |||
| Data output analysis | Support/Optional | |||
| Powe | 0.6kw | 1.1kw | 1.5kw | |
| Size | 420*545*455mm | 510*570*500mm | ||
Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006
Brand:
Yingtai
N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.