8x20ml Angle Rotor 

K-BETA3

SKU: 700004268

100 assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	β-Amylase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	400
Signal Response:	Increase
Limit of Detection:	0.05 U/mL
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 10 min
Application examples:	Cereal flours, malts and other materials.
Method recognition:	Modification of RACI Standard Method

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities.

Advantages

• Very cost effective 
• All reagents stable for > 2 years as supplied 
• Only enzymatic kit available 
• Very specific 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities

Introduction

Intended Use

Used for the selective isolation and culture of Listeria monocytogenes.

Principle and Interpretation

Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and growth factors，maintains balanced osmotic pressure; Listeria hydrolyzes aesculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.

Formulation

Ingredients	/liter
Columbia blood agar base	39.0 g
Aesculin	1.0 g
Ferric ammonium citrate	0.5 g
Lithium chloride	15.0 g
pH7.0±0.2 at 25°C

Preparation

Weigh 55.5 g of this product, add 1000 mL of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15 min. Cool to about 50℃, add 1 bottle of SR0500 supporting reagent A and 1 bottle of B per 100 mL, mix well and set aside.

Quality Control

The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:

Quality control strains	Growth
Listeria monocytogenes ATCC19115	Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212	–
Escherichia coli ATCC25922	–

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use Used for the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Columbia Blood Agar Base provide carbon and nitrogen sources, vitamins and……

endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.