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ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market. 

Originally isolated from Pandalus borealis (Arctic shrimp), it has been purified from a recombinant source since 2010. Unlike other alkaline phosphatases, rSAP can be fully inactivated by a short heat treatment of 15 minutes at 65°C.

This added convenience through complete heat inactivation has made SAP one of the most sold DNA modifying enzymes.

For added flexibility, especially when lyophilisation may be desired, rSAP is also available in a Glycerol FREE format. First launched in 1993, SAP’s unique features continue to make it a valuable tool in various applications.

Key Features 

• Gold standard heat labile, all-purpose alkaline phosphatase.
• Completely inactivated after 5 min at 65°C or 1 min at 75°C.
• Robust: Active in most restriction enzyme buffers, no need for supplemental zinc or other additives for activity.
• Simple, hassle-free dephosphorylation of DNA, RNA, dNTPs and proteins for subsequent use in cloning or end-labelling of probes.
• No vector purification necessary.
• Easy inactivation of unincorporated dNTPs in PCR products prior to DNA sequencing or SNP analysis.
• Excellent stability at 4°C and RT.

Applications

• Dephosphorylation of vectors prior to cloning.
• Enzymatic clean-up of PCR products before sequencing.
• Ideal for MALDI-TOF, cloning, HLA typing, genotyping and sequencing.
• Preparing substrate in protein kinase studies.

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Properties

ArcticZymes’ Shrimp Alkaline Phosphatase (SAP), including its recombinant version (rSAP), is the gold standard and the first heat-labile, all-purpose alkaline phosphatase on the market.

Overview

• Reliable and cost-effective
• Non-invasive, user-friendly sample collection and preservation in one convenient kit
• Preserved RNA is stable for up to 2 months at room temperature
• Compatible with most RNA isolation methods including Norgen’s Saliva/Swab RNA Purification Kits
• High quality RNA is suitable for sensitive downstream applications including RT-PCR, RT-qPCR, RNA sequencing and microarray
• Inactivates viruses including SARS-CoV-2 upon mixing – Explore the Application Note
• Shipping accessories can be purchased separately
• CE-IVD marked version available

Norgen’s Saliva RNA Collection and Preservation Devices are designed for simple and non-invasive saliva collection and preservation of RNA in saliva samples at ambient temperature. Each of the 50 Saliva RNA Collection and Preservation Devices consists of 3 components:

• Saliva Collection Funnel and Collection Tube
• Collection Tube Cap
• Norgen’s Saliva RNA Preservative contained within a sealed squeezable ampoule

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.

Saliva RNA Preservative

Norgen’s Saliva RNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of RNA from fresh specimens. The buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for up to 2 months at room temperature.

Nucleic Acid Isolation from Preservative

Saliva RNA can be isolated from the preserved saliva samples using Norgen’s Saliva/Swab RNA Purification Kits (Cat# 69100, 69300).

Details

Supporting Data

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Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Up to 2 months at room temperature

Shelf Life and Handling

• When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
• Once collected, saliva RNA is stable for up to 2 months when kept tightly sealed and stored at room temperature.
• The Collection Tube, the Collection Funnel and the Device Container of each individual Saliva RNA Collection and Preservation Device are recyclable.

Kit Components	Cat. RU53800 (50 Devices)
Individual Saliva RNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1
Individual Saliva RNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.

NGS FFPE DNA Library Prep Kit Workflow

FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples.  Thus, the extraction of high quality DNA from FFPE samples is often a challenge.  Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.

As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.

Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform:

Non-index (Cat.# 30035): Libraries do not have index.

Index (Cat.# 30037): Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples. Index information can be downloaded here.

Unique dual index (Cat.# 30039): FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system. Our unique Four–Base Difference Index System allows us to make indexes that have at least 4 bases different from each other in the 8-base index sequence. Our unique dual indexing primers can effectively remove NGS errors including index hopping, de-multiplexing errors, index cross-contamination, mis-assignments etc. The unique dual index primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34037).

Kit advantages:

• Fast and simple protocol
  • Making libraries in just 1.5 hours
  • 10 minutes of hands-on time
• Easy procedure
  • Ready-to-use master mix to reduce the tedious mixing
  • Less reaction components to simplify the reaction setup
• Less magnetic beads needed for the purification steps: saving more than 50% of the expensive beads
• Low input FFPE DNA: From 10 ng to 400 ng

Comparison of library conversion efficiency under the same condition. Input DNA amount is 25 ng.

Comparison of library yield under the same condition. Input DNA amount is 25 ng.

The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.