4x100ml Low Speed Angle Rotor 

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments from 100 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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PCR Sizer 100 bp DNA Ladder (Cat# 11400) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

endo-BCN-PEG2-acid is a PEG linker containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-acid is a PEG linker containing a BCN group and a terminal carboxylic acid. The BCN group can react with azide-tagged biomolecules. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.