Propargyl-PEG2-acid is a linker consisting of propargyl and carboxylic acid functional groups. In the presence of activators (e.g. EDC, or HATU), the carboxylic acid reacts with primary amine to form a stable amide bond. The propargyl group reacts with azide group in biomolecules to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG2-acid is a linker consisting of propargyl and carboxylic acid functional groups. In the presence of activators (e.g. EDC, or HATU), the carboxylic acid reacts with primary amine to form a stable amide bond. The propargyl group reacts with azide group in biomolecules to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
RNA Amplification Kit With Improved Detection & Quantification-20°C Format Easy And Efficient
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | RNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
This kit provides a fast and simple spin column procedure for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices (Cat. 49000), as well as fresh saliva samples.
Saliva DNA purified using Norgen’s Saliva DNA Isolation Kit Dx kit is of the highest quality, and can be used with any downstream in vitro diagnostic application employing enzymatic amplification or other enzymatic modifications of DNA followed by signal detection or amplification.
Background
Saliva represents an excellent non-invasive alternative to blood collection. Human genomic DNA extracted from buccal epithelial cells and white blood cells found in saliva can be used in various applications in diagnostics. Saliva DNA can be used for the detection of biomarkers to diagnose a disease, follow the diseases progress or monitor the effects of a particular treatment. Saliva DNA can also be used to diagnose particular types of infections. Isolation of DNA from saliva has become an attractive alternative to isolation from blood or tissue due to the fact that sample collection is non-invasive, the samples can be collected by individuals with little training, and no special equipment is required. Norgen’s Saliva DNA Isolation Kit provides a fast and simple procedure for isolating genomic DNA from both preserved saliva samples and fresh saliva samples.
NOTE: This product is not available for sale in the United States.
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| Kit Specifications | |
| Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
| Average Yield from 0.25 mL of Saliva* | 3 – 7 μg |
| Average Purity (OD260/280) | 1.7 – 2.1 |
| Time to Complete 10 Purifications | 30 minutes |
* Average yield will depending on the donor
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels. The Proteinase K can be stored at either room temperature or 4oC.
| Component | Cat. Dx45400 (50 preps) |
|---|---|
| Lysis Solution | 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL |
| Binding Solution | 12 mL |
| Wash Solution | 18 mL |
| Elution Buffer | 12 mL |
| Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |