Permagen’s most universal ring magnet plate. (without cushion base) From PCR plates to almost any microplate
Available in three magnet strengths depending on your assay or protocol from 350 µL – 2 mL formats
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom, SBS/ SLAS fit on top to accept any microplate
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
U380 / U480 / U500
U380 – Maximum – 350 µL
Minimum – 30 µL
U480 – Maximum – 1 mL
Minimum – 30 µL
U500 – Maximum – 2 mL (Deep Well)
Minimum – 30 µL
Permagen’s most universal ring magnet plate. (without cushion base) From PCR plates to almost any microplate
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases, the gold tagged RNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase is detected). When RNases are present, however, the RNA substrate is cleaved, and the 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases in both liquid and on solid surfaces and a perfect tool for monitoring mRNA vaccine manufacturing.
RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands, and binds the auxiliary protein and single strand With the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology position and strand exchange begins; accompanied by the recombinase from the complex Upon dissociation, the polymerase also binds to the 3′ end of the primer, initiating chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
Parameters | Details |
---|---|
Product Name | RNA Isothermal Amplification Kit NFO |
Manufacturer | Amp-future |
Storage Temperature | -20°C |
Kit Components | Enzymes, Buffers ,Reagents |
Packaging | 48 Tests/box |
Detection Limit | 500-1000copies/µL |
Shipping | ICE |
Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for RNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
RNA NFO kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map