12×10ml Angle Rotor 

Description

The Plant RNA Kit (NA2014-01) is optimized for purification of total RNA from a wide variety of plant samples which rich in polysaccharides and phenol. It comes with proprietary magnetic beads and a specially optimized buffer. Toxic solvents such as phenol, chloroform are not needed in the process. The eluted RNA is high-quality and is ready for all downstream applications, such as RT-PCR, Northern blotting, differential display. The procedures can be fully automated on the magnetic particle processor instrument and ease of use.

Overview

• Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
• Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
• Eliminates PCR inhibitors including humic acids
• High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray
• 96-Well and Magnetic Bead System formats also available

These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.

Stool DNA Isolation Kit (Spin Column)

Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation 96-Well Kit (High Throughput)

Fast and easy high throughput processing using centrifugation. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Stool DNA Isolation Kit (Magnetic Bead System)

Fast and easy processing using a magnetic bead system. Robust lysis system (chemical lysis combined with a mechanical homogenization).

Stool DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)

This kit provides fast and easy processing using a magnetic bead system, and a robust lysis system (chemical lysis combined with a mechanical homogenization). High throughput and compatible with an automation robotic system.

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Extracting Biological Insights from Stool

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Details

Supporting Data

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Kit Specifications
Maximum Stool Input	200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed	Frozen, fresh or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 27600 (50 preps)	Cat. 65600 (192 preps)	Cat. 55700 (50 preps)	Cat. 63100 (192 preps)
Lysis Buffer L	60 mL	2 x 105 mL	60 mL	3 x 60 mL 1 x 30 mL
Lysis Additive A	6 mL	25 mL	6 mL	25 mL
Binding Buffer I	7 mL	25 mL	7 mL	25 mL
Binding Buffer C	30 mL	110 mL	–	–
Wash Solution A	18 mL	2 x 38 mL	–	–
Magnetic Bead Suspension	–	–	1.1 mL	4 x 1.1 mL
Solution WN	–	–	18 mL	55 mL
Elution Buffer B	8 mL	30 mL	8 mL	30 mL
Bead Tubes	50	200	50	196
Mini Spin Columns	50	–	–	–
96-Well Plate	–	2	–	2
Adhesive Tape	–	4	–	2
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	–	–
Elution Tubes (1.7 mL)	50	–	50	–
96-Well Elution Plate	–	2	–	2
Product Insert	1	1	1	1

Description

Specifications

Clone	IHC539
Source	Mouse Monoclonal
Positive Control	Tonsil
Dilution Range	1:200

Cluster of differentiation 57 (CD57), also known as NK-1, is an antigen detectable in natural killer cells, some T-lymphocytes and normal peripheral blood mononuclear cells, myeloid cells, and a variety of polypeptides, lipids, and chondroitin sulfate proteoglycans. CD57 is indicated as a marker for tumors of neuroendocrine origin, including pheochromocytomas, paragangliomas, carcinoid tumor, and medulloblastomas, as well as various neural tumors including neuromas, neurofibromas, schwannomas, and granular cell tumors. CD57 is also detectable in ganglioneuroma and prostate carcinoma. Anti-CD57 is used to distinguish nodular lymphocyte-predominant Hodgkin’s lymphoma from T-cell/histiocyte-rich large B-cell lymphoma, nodular sclerosis Hodgkin’s disease, and follicular lymphoma.