Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The amino group (NH2) is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. Reagent grade, for research use only.

Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The amino group (NH2) is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. Reagent grade, for research use only.

Description

What is the genesig q16?
The genesig q16 is a revolutionary instrument launched by Primerdesign Ltd. The instrument is designed to accompany the genesig Easy product range which includes kits for more than 550 different DNA testing applications. The genesig q16 is designed to make DNA testing affordable and easy for anyone in any business.

What is DNA testing?
DNA testing is the most sensitive and precise way to detect and quantify the presence of a DNA target. The underlying technology within the genesig q16 is real-time quantitative PCR.  The technology has been around for 20 years, but to date has been complex and expensive to perform. The genesig q16 changes all that.

What can I use it for?
The genesig Easy product range includes tests for a massive range of applications:

Incredibly easy to use
Automated data analysis
Robust, beautiful design
Small and portable: 12cm footprint
Over 550 kits available from a vast range of human, food and veterinary detection profiles
Highly portable

Introduction

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA（miRNA）from tissue, cell using two columns and DNase plus reagent
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Products	RNA, miRNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Clinical tissues, cells, lymphocytes
Sample amount	Tissue: <20 mgCells: <5 x 106
Yield	2-50μg
Elution volume	≥30μl
Time per run	≤25 minutes

Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.

Advantages

• Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – the whole extraction only takes 15-25 minutes
• Nontoxic – no toxic phenol chloroform extraction is required in the extraction

Kit Contents

Contents	IVD4121
Purification Times	50 Preps
HiPure DNA Mini Column Ⅱ	50
HiPure RNA Mini Columns	50
2ml Collection Tubes	150
Proteinase K	24 mg
Protease Dissolve Buffer	1.8 ml
DNase I	600 μl
DNase Buffer	6 ml
Buffer RTL	40 ml
RNA Digestion Buffer	15 ml
Buffer RWC*	20 ml
Buffer RW2*	20 ml
Nuclease Free Water	10 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.