This kit provides a rapid spin column method for the purification of total DNA from a broad spectrum of bacteriophages propagated in bacteria grown in liquid cultures. The DNA is isolated without the use of phenol, chloroform or cesium chloride banding procedures. The spin-column based procedure is rapid and can be completed in less than 45 minutes. The kit is highly efficient for processing small volumes of phage supernatant (500 µL – 1 mL) and with the optional DNase and Proteinase K treatments phage DNA yields are maximized while host DNA contamination is minimized. Purified total phage DNA is of the highest integrity, and can be used in a number of downstream applications including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), sequencing, cloning, Southern Blot and more.
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| Kit Specifications | |
| Column Binding Capacity | 50 µg |
| Maximum Column Loading Volume | 650 µL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 1 x 1010 pfu/mL enriched phages |
| Average Yield* | 3-15 µg DNA from 106-1010 pfu/mL of enriched phages |
| Time to Complete 10 Purifications | 45 minutes |
* Average yields will vary depending upon a number conditions used and developmental stage.
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 46800 (50 preps) | Cat. 46850 (100 preps) |
|---|---|---|
| Lysis Buffer B | 40 mL | 2 x 40 mL |
| Wash Solution A | 38 mL | 2 x 38 mL |
| Elution Buffer B | 8 mL | 2 x 8 mL |
| Spin Columns | 50 | 100 |
| Collection Tubes | 50 | 100 |
| Elution Tubes (1.7 mL) | 50 | 100 |
| Product Insert | 1 | 1 |
K-LMALAF
SKU: 700004312
245.5 mL of prepared reagent (e.g. 1116 assays of 0.22 mL)
| Content: | 245.5 mL of prepared reagent (e.g. 1116 assays of 0.22 mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Malic Acid |
| Assay Format: | Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | up to 80 μg/mL of L-malic acid in final reaction solution |
| Limit of Detection: | 20 mg/L |
| Reaction Time (min): | ~ 3 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK |
The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.
View more assay kits on our organic acid list.
Advantages
The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.
N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-Me-N-bis(PEG2-propargyl) has two alkyne groups which can participate in Click Chemistry reactions with azide-bearing compounds or biomolecules; copper is required for catalyzation. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.