This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
| Contents | D635901D | D635902D | D635903D |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles N | 1.1 ml | 2.2 ml | 11 ml |
| Buffer ATL | 40 ml | 80 ml | 2 x 200 ml |
| Buffer BGL | 40 ml | 80 ml | 2 x 200 ml |
| Buffer BST1 | 60 ml | 120 ml | 550 ml |
| Buffer GW2* | 20 ml | 25 ml | 2 x 50 ml |
| Proteinase K | 48 mg | 110 mg | 2 x 220 mg |
| Protease Dissolve Buffer | 5 ml | 10 ml | 30 ml |
| DTT | 235 mg | 235 mg | 3 x 235 mg |
| Elution Buffer | 10 ml | 10 ml | 30 ml |
32/48 channel machine
| Product | Contents and volume | D6359D-TL-06 |
| Buffer BGL | 80 ml | |
| Buffer ATL | 80 ml | |
| Proteinase K | 110 mg | |
| Protease Dissolve Buffer | 10 ml | |
| DTT Powder | 2 x 235 mg | |
| Elution Buffer | 5 ml | |
| AS-Tip | 12 pcs | |
| 2.0ml V bottom plate | Row 1/7：empty | 6 plates |
| Row 2/8：500μl Buffer BST1 | ||
| Row 3/9：500μl Buffer BST1 | ||
| Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul) | ||
| Row 6/12：60μl Elution Buffer |
96 channel machine
| Product | Contents and volume | D6359D-F-96 | D6359D-F-48 |
| Buffer BGL | 80 ml | 40 ml | |
| Buffer ATL | 80 ml | 40 ml | |
| Proteinase K | 110 mg | 48 mg | |
| Protease Dissolve Buffer | 10 ml | 5 ml | |
| DTT Powder | 235 mg | 235 mg | |
| Elution Buffer | 5 ml | 5 ml | |
| 96-Tip (AS) | 1 | 1 | |
| Sample Plate 1 | 500μl Buffer BST1 | 1 | 1 |
| Sample Plate 2 | 500μl Buffer BST1 | 1 | 1 |
| Wash Plate 1 | 20μl MagPure Particles N500μl Buffer GW2 | 1 | 1 |
| Wash Plate 2 | 500μl Buffer GW2 | 1 | 1 |
| Elute Plate | 60μl Elution Buffer | 1 | 1 |
Storage and Stability
Proteinase K and DTT Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.
This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.
| Usage | For selective isolation of Staphylococcus Aureus. |
| How to use | Suspend 111 g in 1 L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121°C for 15 minutes. Cool to 50°C and pour into sterile Petri dishes. |
| Formula (per liter) | Pancreatic digest of casein—————5g Pancreatic digest of animal tissue——5g Beef extract————————————1g D-mannitol————————————-10g Sodium chloride—————————–75g Agar———————————————15g Phenolred——————————–0.025g Final pH———————————–7.4±0.2 |
| Storage | Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years. |
500g
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
The m6A sensitive DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Interested in the analysis of DNA or RNA modifications? Then this DNA polymerase could help to analyze such modifications. The m6A sensitive DNA polymerase exhibits increased misincorporation rates opposite m6A, while unmodified adenine is not affected. This prevents the loss of methylation information during reverse transcription and thus allows direct m6A sequencing. For further information refer to the original publication.