6x8ml PCR Rotor 

Magnetic Beads (tRNA Purification)

Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.

The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.

Workflow without large RNA/DNA contamination

In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.

Workflow with large RNA/DNA contamination

Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos.

Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).

Depletion of larger RNAs. Left panel: depletion of 28S rRNA and 18S rRNA. Right panel: depletion of RNA of 180 nt and larger.

Features

Removal of unwanted components and other impurities

Purification of tRNA and oligos (>70 nt)

tRNA

RNA fragments 70 nt or longer

DNA/RNA hybrid fragments 70 nt or longer

Oligo and chimeric oligo 70 nt or longer

dsDNA fragments 70 bp or longer

ssDNA fragments 70 nt or longer

Removal of larger RNA/DNA contamination:

18S rRNA

28S rRNA

RNA/DNA> 180 nt

Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.

Features:

1. Programmable microprocessor control system, touch screen indicates rotor No., speed, time, temperature, RCF, rate of speed as well as fault information.  

2. Adopts band heater, fast heating speed, temperature uniform, with the function of temperature-control and constant.

3. Centrifugal tube made in special skill, anti-broken when work.

4. Great toque brushless motor, maintenance-free, no powder, fast acceleration and deceleration, low noise, which improve the stability a lot.

5. Electric lid lock, over-speed and over-temperature protection

6.3 phase to keep the best performance.

7. Stainless steel chamber, safe and reliable

TD5B Technical Parameter：

Oil test centrifuge， Heating oil test centrifuge, special tube oil test centrifuge

The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.

Features

• • • Optimized condition for using with the Qubit Fluorometer
    • Uses the Qubit RNA High Sensitivity assay setting
    • Cuts costs by 60%

The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure below).

Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).

Qubit is a registered trademark of Thermo Fisher Scientific.

The kit is developed for RNA quantification. The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL (Figure 1). The RNA Quantification High Sensitivity Kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.