Attogene’s PCR kit for Microcystin is designed for the In vitro analysis of the MycE gene region responsible for assembling part of the Microcystis peptide. The MycE gene region specific primer and probe mix is provided to be detected through the FAM channel on a qPCR machine. A sample of algae is obtained and washed to extract a clean algal gDNA sample. A reaction mixture is assembled from primers, probe, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest; while the DNA probe mixture is cleaved during amplification to release its FAM fluorophore. The resulting FAM release can be detected on a variety of qPCR platforms.
Real time qPCR kit
For screening microcystin gene cluster
Use in combination with Attogene Algae DNA isolation kit
Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-Amine can be used to derivatize carboxyl-containing molecules or activated esters (e.g. The NHS ester) with DBCO moiety through a stable amide bond. The low mass weight will add minimal spacer to modified molecules and the hydrophilic sulfonated spacer arm will greatly improve water solubility of DBCO derivatized molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
Specifications
Features | Specifications |
Main Functions | Recover DNA fragments >100bp from agarose gel(<0.2g), purification of DNA from PCR or enzymatic reaction solution solution |
Applications | PCR, NGS, labeling, ligation and enzyme digestion, etc. |
Purification method | Micro spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Agarose gel, PCR products, enzyme products |
Sample amount | Agarose gel: ≤200mgDNA products: ≤300µl (≤5µg) |
Recovery | 100-5000bp:85%, 5-10kb:60-70% |
Elution volume | ≥10μl |
Time per run | ≤30 minutes(1-24 samples) |
Liquid carrying volume per column | 800µl |
Binding yield of column | 20µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
Contents | D211002 | D211003 |
Purification Times | 100 Preps | 250 Preps |
Buffer GDP | 60 ml | 125 ml |
Buffer DW1 | 40 ml | 90 ml |
Buffer DW2 | 20 ml | 50 ml |
Elution Buffer | 20 ml | 30 ml |
HiPure DNA Micro Columns | 100 | 250 |
2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve
HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 80bp-15kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Note: This kit can only recover 200mg of gel at most. If it is larger than 200mg, the column may be blocked. This column can only recover 5μg of DNA at most.
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