40nm Colloidal Gold for Lateral Flow is a highly stable and uniform 40 nm gold nanoparticles can be supplied in a range from 1 OD to 100 OD. The quality and performance of a conjugate is critical to successful lateral flow test manufacturing. Our products are made in USA and produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
Bulk pricing and manufacturing supply contracts available.
Functionally Tested in Lateral Flow
Specifications 1OD 40nm Gold (1L)
| Number of particles/mL | 5.2-7.2 x 1010 |
| Gold Concentration (mg/mL) | 3.7-4.2 x 10-2 |
| Molar Concentration (moles/liter) | 8-12 x 10-11 |
| Particle Diameter | 40 nm /- 1.5 |
Number of particles/mL 5.2-7.2 x 1010
• Gold Concentration (mg/mL) 3.7-4.2 x 10-2
• Molar Concentration (moles/liter) 8-12 x 10-11
• Particle Diameter 40 nm /- 1.5
The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from <150 mg simple plant sample without chloroform |
| Applications | RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology, DNA filtration technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic crops |
| Sample amount | ≤150 mg |
| Elution volume | ≥30μl |
| Time per run | ≤25 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Advantages
Kit Contents
| Contents | R415102 | D415103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure DNA Mini Column II | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer PRC1 | 50 ml | 200 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Select the right purification kit to get impactful results：
Ⅰ. Purchase guide of Magen kits based on sample
Ⅱ. Cross purchase guide for Magen kits vs Other brands
For any customized product or OEM service, please contact us.
The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
