Mal-amido-PEG5-alkyne is a PEG linker containing a maleimide group and an alkyne. The hydrophilic PEG spacer increases solubility in aqueous media. The alkyne group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Mal-amido-PEG5-alkyne is a PEG linker containing a maleimide group and an alkyne. The hydrophilic PEG spacer increases solubility in aqueous media. The alkyne group can react with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
The PM2610/PM2611 ExcelBand™ Enhanced 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand™ Enhanced 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, the PM2610/PM2611 Enhanced 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM2610/PM2611 ExcelBand™ Enhanced 3-color High Range Protein Marker is a ready to use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine buffer (9 to 235 kDa in BisTris (MOPS) buffer and 10-235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand™ Enhanced 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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| Kit Specifications | |
| Minimum Blood Input | 20 µL |
| Maximum Blood Input | 200 µL |
| Column Binding Capacity | > 50 µg |
| Average Yield (200 µL of blood) | 4-12 µg* |
| Time to Complete 10 Purifications | 30 minutes |
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
|---|---|---|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | – | – | – | – | – | 2 x 1.1 mL | 8.5 mL |
| Maxi Spin Columns | – | – | – | 12 | – | – | – |
| Mini Spin Columns | 50 | 100 | – | – | – | – | – |
| Midi Spin Columns | – | – | 20 | – | – | – | – |
| 96-Well Plate | – | – | – | – | 2 | – | 2 |
| Adhesive Tape | – | – | – | – | 4 | – | 2 |
| Collection Tubes | 50 | 100 | 20 | 12 | – | – | – |
| 96-Well Collection Plate | – | – | – | – | 2 | – | – |
| Elution Tubes | 50 | 100 | 20 | 12 | – | 50 | – |
| 96-Well Elution Plate | – | – | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |