Introduction

MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

Details

Specifications

Features	Specifications
Main Functions	Rich small fragment cfDNA from 0.6ml serum plasma, remove DNA fragments＞500bp
Applications	qPCR, NGS, etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Serum, plasma
Sample amount	0.6ml
Elution volume
Time per run

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysedand digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, Large DNA(>500bp) will be adsorbed on the surface of magBindparticles. After removal of large size, small size of DNA(<500bp) will be adsorbed on the surface ofMagPure Particle F and impurities such as proteins will be removed without adsorption. The adsorbedparticles were washed with washing solution to remove proteins and impurities, washed with ethanol toremove salts, and finally DNA

Advantages

• Economy – less than 50% of the price of Qiagen and other imported products
• Automatic – without labour

Kit Contents

Contents	1292750	12927200
Purification Times	50 preps	200 preps
MagPure Particles F	1.2 ml	4.5 ml
MagBind Particles	1.2 ml	4.5 ml
Proteinase K	24 mg	90 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer MLB	30 ml	120 ml
Buffer GDP	15 ml	60 ml
Buffer MAW1	40 ml	250 ml
Buffer MW2*	20 ml	50 ml
Elution Buffer	15 ml	60 ml

Storage and Stability

Proteinase K, MagBind Particles and MagPure Particles F should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

MagPure Circulating DNA Rich Kit designed for purification of high quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, biochip analysis and NGS.

DDL5 Low Speed 5000RPM Swing out rotor 4x800ml Refrigerated Centrifuge Adapters for 5/7/10/15/50ml tubes.

DDL5 Refrigerated Centrifuge Application:

DDL5 Refrigerated centrifuge widely used in the field of blood bank station, pharmaceutical factory and laboratory, can hold 500ml triple, 400ml triple and 250ml triple blood bags. 

DDL5 Refrigerated Centrifuge Features:

1. Brushless frequency motor which in great torque, free maintenance, no powder pollution, quick in speed up and down.

2. Digital display which indicates the program, speed, time, RCF and temperature.

3. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration/deceleration for your choice.

4. There are 6 kinds of rotors for your choice.

5. Electric lid lock, compact design, super speed and imbalance protection.

6. The centrifuge body is made of high-quality steel, safe and reliable.

DDL5 Refrigerated Centrifuge Technical Parameters:

Max Speed:5000r/min	Max  RCF:4730xg
Max Volume；4x800ml	Power supply:AC220V,50HZ,7A
Timer:1~9h/59min	Noise:≤65dBA
Dimension:720x540x830mm	Net Weight: 170KG
Speed Accuracy:±20r/min	Package (W*D*H):850×650×1280
Temperature range:	-20℃ ~ 40℃

DDL5 Refrigerated Centrifuge Matched Rotors:

Order No.	Rotor type	Max.Speed (rpm)	Max.Volume (ml)	Max.RCF (xg)
No30671	Swing out rotor	4,000	4x800ml(round cup)	3,450
No30679	Swing out rotor	4,000	4x500ml(square cup)	3,450
No30696	Swing out rotor	4,000	4x500ml(round cup)	3,530
No31377	Swing out rotor	5,000	4x50ml	4,730
			4x100ml	4,730
No30592	Swing out rotor	4,000	4x30x7ml	3,140
			4x40x7ml	3,270
			4x18x10ml	3,140
No31491	Mircoplater rotor	4,000	2x4x96well	2,910
No31494	Microplater rotor	4,000	4x4x96well	2,940
No30627	Angle rotor	5,000	30x15ml	3,830
No30641	Angle rotor	5,000	12x50ml	3,860
No30642	Angle rotor	4,000	24x50ml	2,970
No30614	Angle rotor	5,000	6x100ml	3,130
No30643	Angle rotor	4,000	12x100ml	2,970

The Corners of our Company____________________________________

Overview

• Detection kits for ZIKV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 4

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Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM62250 (100 preps)	Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
ZIKV Primer & Probe Mix	280 μL	280 μL
ZIKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1