endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from bacteria culture
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Bacteria culture
Sample amount	Bacteria: <10^9
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 30 minutes
• High purity – the purified RNA can be directly used in various downstream applications
• High recovery – RNA can be recovered at the level of PG
• Good repeatability – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried lysozyme and glass beads

Kit Contents

Contents	R418101	R418102	R418103
Purification Times	10 Preps	50 Preps	250 Preps
gDNA Filter Mini Columns	10	50	250
HiPure RNA Mini Columns	10	50	250
2ml Collection Tubes	20	100	500
Glass Beads (0.1-0.6mm)	10 g	30 g	150 g
Plastic spoon	2	4	10
Lysozyme	20 mg	90 mg	400 mg
Protease Dissolve Buffer	1.8 ml	1.8 ml	10 ml
Buffer TE	1.8 ml	1.8 ml	5 ml
Buffer STL	5 ml	20 ml	90 ml
Buffer RLC	10 ml	30 ml	150 ml
Buffer RW1	10 ml	50 ml	250 ml
Buffer RW2*	5 ml	20 ml	2 x 50 ml
RNase Free Water	1.8 ml	10 ml	30 ml

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has  you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.

ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.

Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

A040523

Features

• ANSI/ SBS footprint
• Compatible with 384-well and 96-well plates
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Made in USA 
• For Research use only

Compatibility

• 384-well pcr plates
• Some 384-well deep well plates
• Full-, Semi-, and un-Skirted 96-well PCR Plates
• Any magnetic beads
• Any common liquid handlers i.e. Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

384-Well PCR

Maximum – 39 µL 

Minimum – 5 µL 

__________

96-Well PCR

Maximum – 60 µL (0.2 mL for some beads)

Minimum – 4 µL

Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 5 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.