Propargyl-PEG8-acid has an alkyne group and an acid group. The acid group can derivatize amine-containing molecules in the presence of activators such as EDC or HATU. The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-acid has an alkyne group and an acid group. The acid group can derivatize amine-containing molecules in the presence of activators such as EDC or HATU. The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sample type purification kit guide
The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
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| Minimum amount of starting material: | 2.5 µL of DNA (5 ng/µL) |
| Time to complete library preparation: | 4 hours |
Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
| Step | Component | Cat. 70100 (24 preps) | Cat. 70110, 70120, 70130, 70140 (96 preps) |
|---|---|---|---|
| Amplicon PCR (PCR 1) | MGX Master Mix | 330 µL | 1,320 µL |
| 16S V1-V2 Primer Mix | 70 µL | 280 µL | |
| Index PCR (PCR 2) | Indexing Master Mix | 660 µL | 2 x 1,320 µL |
| N7xx Index Primer | 50 µL | 50 µL | |
| S5xx Index Primer | 70 µL | 70 µL | |
| PCR Clean-Up | Resuspension Buffer | 2 x 1,250 µL | 2 x 5,000 µL |
| Nuclease-free water | 1,250 µL | 1 x 6,000 µL |
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Specifications
| Features | Specifications |
| Main Functions | Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid. |
| Applications | Real Time PCR, biochip analysis, NGS |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Sample amount | 0.5 – 1.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
| Contents | R667200C | R667202C |
| Purification Times | 24 Preps | 96 Preps |
| 2ml Bead Tube (0.4g) | 24 | 96 |
| DNase I (Powder) | 10 mg | 15 mg |
| DNase Buffer | 5 ml | 20 ml |
| Protease Dissolve Buffer | 3 ml | 8 ml |
| Lysis Buffer LBX1 | 40 ml | 180 ml |
| Buffer TL | 5 ml | 20 ml |
| Proteinase K | 24 mg | 120 mg |
| MagBind Particles N9 | 1.2 ml | 5 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer MW1* | 13 ml | 110 ml |
| Buffer MW2* | 10 ml | 50 ml |
| Buffer AVE | 10 ml | 20 ml |
Storage and Stability
Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Product Highlights
Speed: Adjustable speed, up to 4500 rpm
Vortex Oscillation Intensity:1-4 intensity levels, selectable and adjustable
Programming:Programmable,freely choose combinations of oscillation strength,duration.centrifugation speed, and time
Safety:Safety interlock device to ensure user safety
Compatibility: Single tube or multi-tube centrifugation/mixing with one-button operation, saving time and cost
Display:Touch buttons and an LCD screen clearly indicate and set oscillation level, duration, and cycle count
Convenience: Multi-functional, compact design, occupies minimal space
Technical Specifications
| Dimensions (mm) | 200(w)x243(H)x127(D) | Working temperature | 5℃ to 40℃ |
| Oscillation Duration | 0-30 seconds (increment of 1 second) | TD4Z Speed range | 1000-3600 rpm (increment of 100 rpm) |
| Power Supply | AC 220V 50/60 Hz | Spin Timer | 0 seconds to 30 minutes |
| Weight | 3.5kg (including power supply) | Oscillation Intensity | Level 1-4 |
| Speed range TD4Z | 1-99 programs | Centrifugation Adjustment TD4Z | 1000-3600 rpm (increment of 100 rpm) |
PCR laboratories and general molecular biology laboratories
Model:
TD4Z
Brand:
Yingtai
Availability: Pre Order
