Propargyl-PEG2-NHS ester is a PEG linker with a propargyl group that can participate in copper catalyzed azide-alkyne Click Chemistry and NHS ester that can be used to label amine-containing entitites.
Propargyl-PEG2-NHS ester is a PEG linker with a propargyl group that can participate in copper catalyzed azide-alkyne Click Chemistry and NHS ester that can be used to label amine-containing entitites.
Propargyl-PEG2-NHS ester is a PEG linker with a propargyl group that can participate in copper catalyzed azide-alkyne Click Chemistry and NHS ester that can be used to label amine-containing entitites.
The CloneSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our CloneSizer contains fourteen discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments, double intensity reference bands at 500 and 1000 bp and an additional 2686 bp (pUC19) reference band for easy clone identification.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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CloneSizer 100 bp DNA Ladder (Cat# 11600) – 100 loads
Ladder Properties:
| Fragment | Size (bp) | Mass (ng) |
| 1 | 2686 | 72 |
| 2 | 2000 | 53 |
| 3 | 1500 | 41 |
| 4 | 1200 | 42 |
| 5 | 1000 | 56 |
| 6 | 900 | 30 |
| 7 | 800 | 29 |
| 8 | 700 | 25 |
| 9 | 600 | 25 |
| 10 | 500 | 52 |
| 11 | 400 | 19 |
| 12 | 300 | 20 |
| 13 | 200 | 19 |
| 14 | 100 | 17 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
This kit is designed for the rapid preparation of genomic DNA from various tissue samples, cultured cells, viruses, bodily fluids and swabs using a rapid spin column protocol. Purified DNA is of an excellent yield and quality, and is immediately ready for any downstream application including PCR, qPCR, genotyping, sequencing and more. The protocol can be completed in approximately 80 minutes (including incubation time).
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| Kit Specifications | |
| Column Binding Capacity | 25 µg |
| Average Yield:* HeLa Cells (1 x 106) Tissue (from 10 mg kidney) | 8 µg 10 µg |
| Maximum Amount of Starting Material: Animal Tissues Cultured Cells Bodily Fluids (blood, saliva) Viral Suspension | 20 mg 3 x 106 cells 150 µL 150 µL |
| Time to Complete 10 Purifications | 80 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 24700 (50 preps) | Cat. 24750 (100 preps) | Cat. 24770 (250 preps) |
|---|---|---|---|
| Digestion Buffer A | 25 mL | 2 x 25 mL | 5 x 25 mL |
| Buffer SK | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 5 x 18 mL |
| Elution Buffer B | 30 mL | 2 x 30 mL | 5 x 30 mL |
| Proteinase K | 12 mg | 2 x 12 mg | 5 x 12 mg |
| Spin Columns | 50 | 100 | 250 |
| Collection Tubes | 50 | 100 | 250 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 |
| Product Insert | 1 | 1 | 1 |
