Propargyl-PEG3-NHS ester

Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.

Lateral flow strips designed to detect a biotin and FITC/FAM labelled amplicon. Milenia Genline HybriDetect 1 (PDF 276 KB) lateral flow strips can be used to detect amplification products generated when using a TwistAmp® nfo kit in combination with a TwistAmp® nfo probe and labelled amplification primer.

Introduction

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

Details

Specifications

Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.

After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

Ordering information

Purchase Guide

• The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

Opentrons OT-2 Filter Tips, 200µL. Optimized for volumes 20µL to 200µL. Clear Tips, Polypropylene tip, Polyethylene Filter Mesh, RNase-/DNase-free, Nonpyrogenic, Protease free. Pre-sterilized with electron beam irradiation. Not Autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.

Opentrons OT-2 Filter Tips, 200µL. Optimized for volumes 20µL to 200µL. Clear Tips, Polypropylene tip, Polyethylene Filter Mesh, RNase-/DNase-free, Nonpyrogenic, Protease free. Pre-sterilized with electron beam irradiation. Not Autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN2 P300 Electronic Pipettes, Opentrons Single-Channel GEN1 P50 Electronic Pipettes, Opentrons Single-Channel GEN1 P300 Electronic Pipettes, Opentrons 8-Channel GEN1 P50 Electronic Pipettes, Opentrons 8-Channel GEN1 P300 Electronic Pipettes.