Propargyl-PEG4-CH2CO2-NHS has a propargyl group and an NHS group. This reagent is amine reactive, thus, useful for derivatizing biomolecules with amine group. The propargyl group reacts with azides via copper catalyzed azide-alkyne Click Chemistry to form stable triazole bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-CH2CO2-NHS has a propargyl group and an NHS group. This reagent is amine reactive, thus, useful for derivatizing biomolecules with amine group. The propargyl group reacts with azides via copper catalyzed azide-alkyne Click Chemistry to form stable triazole bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.
DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
endo-BCN-PEG3-Gly represents an ADC linker featuring a BCN group, a hydrophilic PEG3 linker, and glycine residue. The BCN group is a click chemistry handle that readily reacts with azide groups on a target molecule to form stable triazole linkages. The PEG units enhance the aqueous solubility of the compound and may assist in fine-tuning DMPK properties. This compound may be applied to the synthesis of antibody-drug conjugates.
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
Specifications
| Features | Specifications |
| Main Functions | Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture |
| Applications | Enzyme digestion, sequencing, PCR and labeling, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional plasmid, plasmid≤30KB |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤80 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | P181402 | P181403 | P181404 |
| Purification Times | 100 Preps | 500 Preps | 5000 Preps |
| RNase A | 10 mg | 30 mg | 2 x 160 mg |
| Buffer P1 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer P2 | 30 ml | 150 ml | 2 x 800 ml |
| Buffer LEN3 | 20 ml | 80 ml | 800 ml |
| Buffer LN4 | 90 ml | 400 ml | 4 x 980 ml |
| MagPure Particles | 3.5 ml | 17 ml | 3 x 60 ml |
Storage and Stability
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. Theremaining kit components can be stored dry at room temperature (15–25°C) and are stable for atleast 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature whenused. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.
