Propargyl-PEG7-t-butyl ester

Overview

• Detection kits for Cryptococcus neoformans
• Available in TaqMan format for analysis

Cryptococcus neoformans is an encapsulated yeast. Infection with C. neoformans is known as cryptococcosis and is the cause of the most common life-threatening meningitis in patients with weakened immune systems, particularly in advanced HIV/AIDS. C. neoformans is commonly found in soil throughout the world. Human infection of C. neoformans occurs via inhalation of aerosolized spores. From the lungs, C. neoformans is spread hematogenously to the Central Nervous System (CNS), resulting in meningoencephalitis. Although the availability of antiretroviral therapy in the developed world has reduced the incidents of cryptococcosis, it is still a major problem in developing countries and is one of the leading causes of death in patients with HIV/AIDS. In fact, one of the major challenges in treating cryptococcosis is that many patients with cryptococcal CNS disease are asymptomatic in terms of cryptococcal pneumonia, making it difficult for early detection.

Cryptococcus neoformans TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Cryptococcus neoformans TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM42750 (100 preps)	Cat. TM42710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Cryptococcus neoformans Primer & Probe Mix	280 μL	280 μL
Cryptococcus neoformans Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Product Description

20mins High Sensitivity Isothermal DNA Amplification Kit For Accurate Detection

Product Detail

Applicable equipment:

It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit EXO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(fluorescent type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Fluorescent Probe:Require the suitable length is 46-52nt.

DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Overview

• Convenient optimized on-column DNase treatment using Norgen’s RNA Purification Kits
• Also includes protocol for digestion in-solution followed by RNA Clean-Up
• Guaranteed RNase-Free
• Includes Enzyme Incubation Buffer
• Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

Details

Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

Storage Conditions
The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8ºC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20ºC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

Component	Cat. 25710 (50 rxns)	Cat. 25720 (200 rxns)
DNase I	1 vial / 1,600 units	4 vials (1,600 units/vial)
Buffer DR	1 mL	4 x 1 mL
Enzyme Incubation Buffer	6 mL	4 x 6 mL
Product Insert	1	1