Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
NGS Cell Free DNA Library Prep Kit Workflow
The main source of cell-free DNA (cfDNA) is derived from apoptotic hematopoietic cells in blood and found in the plasma. The length of the cfDNA is about 150-200 bp in length. Circulating tumor DNA (ctDNA) derived from malignant tumors is a part of cfDNA. Both cfDNA and ctDNA can be used as a noninvasive biomarker since it offers a better approach in comparison to invasive tissue biopsies.
NGS has been used for cfDNA and ctDNA sequencing in the field of liquid biopsy as it provides a whole genome level of molecular profiling. One of the hurdles for cfDNA sequencing is the difficulty of library preparation from the limited amount of cfDNA obtained from plasma. Our cell-free NGS kit makes it easy to get enough libraries from limited input in just 1.5 hours.
Three index types are available for the NGS Cell Free DNA Library Prep Kit of the illumina platform:
Non-index (Cat.# 30029): Libraries do not have index.
Index(Cat.# 30031): Each of our index primers contains a unique 6-base index sequence that can be used for sample identification. Total 48 library multiplexing is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30033): Cell-free DNA library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a Four-Base Difference Index System. The system have at least 4 bases different from each other in the 8 bases index length. The primers effectively minimize sequencing errors such as mis-assignment, index hopping, index contamination etc. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34031).
Kit advantages:
Comparison of library conversion efficiency with different samples under the same condition.
Comparison of library yield with different samples under the same condition.
The NGS Cell Free DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality cell-free DNA (cfDNA) libraries using 1 ng to 50 ng of cell-free DNA as input. The kit has a simple work flow and a fast procedure. Multiplexing of the cell free DNA library is possible based on the index type.
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
The kit uses linear dsDNA libraries (MGI platform) as input and enriches the circularized single-stranded DNA libraries. The circularization kit has a higher library circularization efficiency (25%) as compared to other vendors (7-15%).
NGS Library Circularization Kit workflow
Comparison of Library Circularization Efficiency
Kit features
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
Character of Yingtai laboratory scale freeze dryer
Small size, and light weight
Compact construction of tabletop freeze dryer
Suitable for small samples drying
Advantage of Yingtai 3kgs smallest lab scale freeze dryer
3kg water capture capacity, which can meet the needs of general laboratory freeze-drying capacity
-70°C ultra-low temperature cold trap, which can capture conventional organic solvents
Direct-capture coil cold trap with higher efficiency and better ice-catching effect
The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry organic solvents
Large flat flange type silicone seal, effectively guarantee the vacuum tightness, no need to apply any auxiliary sealing silicone grease forever
One-button operation, the host and the vacuum pump are linked, no manual operation required
The Pirani vacuum sensor with automatic temperature compensation can accurately detect the vacuum degree in the chamber at each stage
Removable sample shelf with special holes for centrifuge tubes, making it easier to place and adjust the height
Various types of chambers and T-frame configurations can fully meet the user’s sample placement
Vacuum pumps and sample placement chambers can be freely combined according to user needs, making the configuration more flexible and convenient
Techinical parameter
| Item No | BD1-3 | BD1-6 | BD2-6 |
| 24/h water capture capacity | 3kg | 6kg | 6kg |
| Cold trap temperature | -70℃ | -70℃ | -90℃ |
| Cold trap volume | 5L | 10L | 10L |
| Freeze dried chamber | High permeability organic glass/multi manifold external valve/stainless steel chamber | ||
| Sample shelf | φ 200mm stainless steel with 3-5 layers available | ●Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional | |
| vacuum pump | Standard configuration | ||
| Oil mist filter | Extreme vacuum 4 * 10-4mbar,with a pumping capacity of 78L~200L/optional pump of various types | ||
| Vacuum pump connection pipe | Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m | ||
| anti-corrosive treatment | Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents | ||
| External valve | 6/optional | 1-48 pieces/optional | |
| Eutectic point detection system | Support/Optional | ||
| Data output analysis | Support/Optional | ||
| Power | 0.6kw | 1.1kw | 0.5kw |
