Introduction

This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.

Details

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.

Kit Contents

Contents	R662601	R662602	R662603
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure RNA Particles	1.7 ml	4.0 ml	18 ml
DNase I	600 μl	2 x 600 μl	10 x 600 μl
DNase Buffer	30 ml	40 ml	200 ml
Buffer SPL	30 ml	60 ml	270 ml
Buffer PHC	30 ml	60 ml	270 ml
Buffer MCB*	9 ml	15 ml	75 ml
Buffer ALB3*	10 ml	20 ml	100 ml
Buffer GW1*	44 ml	66 ml	2 x 220 ml
Buffer RW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	30 ml	120 ml
2ml Bead Tubes	48	96	5 x 96

Storage and Stability

MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is specially designed for stool RNA extraction. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Ideal for use in in vitro workflows
• No need to immediately process samples
• Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
• Ship stool samples at room temperature safely
• Compatible with most DNA/RNA isolation methods 

Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx – 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

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Kit Specifications
Stool Input	2 g
Stability of Stool Nucleic Acids at Room Temperature	2 years for DNA 7 days for RNA*

* The RNA stability will be vary depending on the samples

Stool Nucleic Acid Collection and Preservation Devices Dx – 50 Contents:

Kit Components	Cat. Dx45660
Stool Nucleic Acid Collection and Preservation Devices Dx	50
Product Insert	1

Stool Nucleic Acid Collection and Preservation Device Dx Contents:

Kit Components	Contents
Stool Nucleic Acid Collection and Preservation Tube Dx filled with preservative	1
Insert Card	1

Description 

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users. 

Features

• Cloning efficiency greater than 90% 
• IPTG and X-Gal are not required 
• Accepts a wide range of insert/vector ratios 0.5:1 to 12:1 
• Accepts insert size from 6 bp to 11 kb
• The phosphorylation of PCR fragments is not required
• Accepts blunt end amplicon or DNA fragment (not for sticky ends)
• Ampicillin and kanamycin selection markers

Storage

-20°C for 24 months

The GetClone™ PCR Cloning Vector II is a positive selection system for high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation of a blunt end DNA insert into the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/white screening. This cloning vector includes ampicillin and kanamycin resistance genes that can meet the needs of most users.