Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-alcohol is a heterobifunctional PEG linker which enables Click Chemistry reactions with azide-bearing biomolecules; copper catalyst is needed. The hydrophilic PEG units increase aqueous solubility. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-ASNAM
SKU: 700004266
100 assays (50 of each) per kit
| Content: | 100 assays (50 of each) per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 6 months under recommended storage conditions |
| Analyte: | Ammonia, L-Asparagine, L-Glutamine |
| Assay Format: | Spectrophotometer, Microplate |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 0.2 to 7.0 µg of ammonia, or 0.5 to 50 μg of L-asparagine or L-glutamine per assay |
| Limit of Detection: | 0.50 mg/L (L-asparagine), 0.54 mg/L (L-glutamine), 0.06 mg/L (ammonia) |
| Reaction Time (min): | ~ 20 min |
| Application examples: | Potatoes, potato products, vegetables, cereals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Novel method |
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Browse our complete list of assay test kits.
Advantages
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Description
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM2600 ExcelBand™ 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months
-20°C for long term storage
The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG8-NHS Ester is a click chemistry molecule cosisting of an NHS ester that is reactive specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG8 spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
