Propargyl-PEG10-alcohol

Overview

• Robust Lysis Solution processes even the most challenging plant species such as pine needle and grape 
• No phenol extractions
• DNA and all sizes of RNA are recovered, including microRNA
• High quality DNA and RNA are purified simultaneously using the same spin column
• No need to split the lysate​
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides for rapid spin column isolation and purification of total RNA and genomic DNA simultaneously from a single plant sample without splitting the lysate. Norgen’s plant lysis solution is highly robust and effective over a wide range of plants including challenging samples.  The total RNA and genomic DNA are both column purified in under 30 minutes.   Since RNA and DNA are isolated without splitting the lysate, variability and inconsistent results are reduced.​​ All sizes of RNA including microRNA are recovered without the need for phenol.  Optional on-column DNase and RNase treatments provide flexibility to isolate DNA-free RNA or RNA-free DNA respectively.  Isolated nucleic acids are of a high quality and yield, and are ready for downstream use including PCR, qPCR, RT-PCR, qRT-PCR, sequencing and more. 

Background

It is often necessary to isolate total RNA and genomic DNA from a single plant sample, such as for studies of gene expression, mutant or transgenic plant characterization, and host plant-pathogen characterization. This is of great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more reliable since the RNA and DNA are derived from the same sample, therefore eliminating inconsistent results.

Details

Supporting Data

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Kit Specifications
Column Binding Capacity	50 μg for RNA 15 μg for genomic DNA
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material: Plant Tissues Plant Cells	100 mg 5 x 106
Time to Complete 10 Purifications	30 minutes
Average Yields* Peach Leaves (100 mg)	40 μg RNA, 5 μg gDNA

* Yield will vary depending on the type of sample processed

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.

Component	Cat. 24400 (50 preps)
Lysis Buffer M	40 mL
Binding Buffer I	7 mL
Solution WN	18 mL
Wash Solution A	38 mL
Elution Buffer E	20 mL
Enzyme Incubation Buffer B	6 mL
Filter Columns	50
Spin Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	50
Product Insert	1

Product advantages

1. The instantaneous centrifugal function rises to the maximum speed for about 6 seconds, allowing for rapid detachment of hanging droplets from the wall;

2. Faster acceleration and deceleration rate, required to complete the experiment in a shorter time;

3. Door cover protection, overspeed and imbalance detection system, capable of real-time monitoring of  the centrifugal process, ensuring the safe operation    of the  instrument; When the operation ends, an error occurs, or an imbalance occurs, the sound signal prompts, and the operation stops at the same time. The LCD displays the result code

Host Parameters

Product model	Mini-3
Input power supply	DC24V/2.75A
Input power	55W
Motor/Drive Method	DC24V brushless DC variable frequency motor
Display method	High brightness LCD screen
Effective centrifugal time	1-99min.1-59sec
speed	300~3000±15rpm
Speed step increase	10rpm
Maximum capacity	2 *96 well PCR plates/ELISA plates
MAX. RCF	608 xg
Misoperation/alarm failure	Sound prompt+display code
weight	3.9Kg
Noise	60dB (A)
Fastest acceleration time	<6s
Fastest deceleration time	<5s
Permissible ambient temperature/relative temperature	+5-40°C/80%

The micro porous plate centrifuge adopts international advanced design concepts and manufacturing technology, with a smooth and beautiful appearance, compact and stable structure, adhering to the advantages of high universality and easy operation. It also has the functional characteristics of “smooth start” and “smooth braking”; This micro porous plate centrifuge is very suitable for 96 or 384 well and small capacity micro porous plates, as well as various standard PCR micro porous plates with or without skirts .

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request